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Lapin anti-Souris IgG Anticorps (PE)

IHC, FACS, IF Polyclonal PE
N° du produit ABIN1967823
  • Antigène Tous les produits IgG
    IgG
    Reactivité
    • 687
    • 681
    • 568
    • 442
    • 378
    • 235
    • 199
    • 156
    • 138
    • 130
    • 113
    • 97
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    • 3
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    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Hôte
    • 1688
    • 1473
    • 548
    • 243
    • 104
    • 90
    • 33
    • 30
    • 21
    • 16
    • 15
    • 11
    • 11
    • 1
    Lapin
    Clonalité
    • 3432
    • 196
    • 1
    Polyclonal
    Conjugué
    • 574
    • 401
    • 393
    • 351
    • 276
    • 245
    • 191
    • 119
    • 98
    • 66
    • 64
    • 61
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    • 21
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    • 17
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    • 15
    PE
    Application
    • 1776
    • 1713
    • 1690
    • 1443
    • 934
    • 910
    • 883
    • 463
    • 243
    • 243
    • 233
    • 100
    • 92
    • 85
    • 76
    • 50
    • 50
    • 32
    • 31
    • 27
    • 24
    • 21
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    • 11
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    • 5
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    • 2
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    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
    Specificité
    The antibody shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
    Purification
    Immunoaffinity purified
    Immunogène
    Anti-Mouse IgG subclass pan reactive Secondary Antibody was produced by repeated immunization with highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins.

    Type of Immunogen: Purified protein
  • Indications d'application
    Approved: Flo, IF (1:100 - 1:250), IHC

    Usage: Anti-Mouse IgG subclass pan reactive Secondary Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
    Commentaires

    Target Species of Antibody: Mouse

    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    Distilled water
    Concentration
    Lot specific
    Buffer
    0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA
    Conseil sur la manipulation
    avoid freeze thaw cycles
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
  • Antigène
    IgG
    Abstract
    IgG Produits
    Classe de substances
    Antibody
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