Chèvre anti-Humain IgA, IgG, IgM (Fc Region) Anticorps (FITC)

N° du produit ABIN2622479

Détail du produit

Antigène: IgA, IgG, IgM

Épitope: Fc Region

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: FITC

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Human IgG, IgA and IgM, Fc specific.

Purification: Purified IgG

Immunogène: Purified polyclonal human IgG, and IgA and IgM containing fractions isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Type of Immunogen: Purified protein

Information d'application

Indications d'application: Approved: ELISA, ICC, IF, IHC, IHC-Fr

Usage: The reactivity of the antiserum is directed to the Fc subunits of the major isotypes of the human immunoglobulin system. No reaction is obtained with purified IgG/Fab or any non-Ig protein of human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal human plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates. to demonstrate immunoglobulins or specific antibodies in cells and tissues. to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen or immune complex using a reference antibody of human origin in the middle layer of the indirect test procedure. The absence of activity to the common Ig/Fab subunit prevents the reaction of this conjugate with immunoglobulins bound to Fc receptors on non-lymphoid cells. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:120. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2. No preservative added.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détail du antigène

Antigène: IgA, IgG, IgM

Autre désignation: IgA + IgG + IgM (IgA, IgG, IgM Produits)

Classe de substances: Antibody

Sujet: This antibody detects: IgA + IgG + IgM Fc