Chèvre anti-Humain IgA, IgG, IgM Anticorps (FITC)

N° du produit ABIN2622482

Détail du produit

Antigène: IgA, IgG, IgM

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: FITC

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Dot Blot (DB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Human IgG, IgA and IgM, heavy and light chains.

Purification: Purified IgG

Immunogène: Purified polyclonal human IgG, and IgA and IgM containing fractions isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Type of Immunogen: Purified protein

Information d'application

Indications d'application: Approved: DB, ELISA, ICC, IHC, IHC-Fr

Usage: The reactivity of the antiserum is directed to the major isotypes of the human immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal human plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates. to demonstrate immunoglobulins or specific antibodies in cells and tissues. to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen or immune complex using a reference antibody of human origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2. No preservative added.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détail du antigène

Antigène: IgA, IgG, IgM

Autre désignation: IgA + IgG + IgM (IgA, IgG, IgM Produits)

Classe de substances: Antibody

Sujet: This antibody detects: Human IgA + IgG + IgM