The RG7/7.6 antibody reacts strongly with rat Igs bearing - light chain of the Igk-1[a]allotype (e.g., AO, AUG, BN, BUF, LEW, LOU, PVG, WAG, WF), weakly with Igk-1[b] light chains (e.g., ACI, COP, DA, F344, SHR), and it does not react with λ light chain nor heavy chains. At the time that this antibody was characterized, the Igk-1[a] allotype was designated - 1b, and Igk-1[b] was -1a, the current nomenclature was standardized at the Fourth International Workshop on Allogenic Systems in the Rat. The RG7/7.6 antibody also strongly cross-reacts with Syrian and Armenian hamster Ig - light chain.
BD Pharmingen™ Biotin Mouse Anti-Rat and Anti-Hamster Ig, κ Light Chain - Biotin - Clone RG7/7.6 - Isotype Mouse IgG2a, κ - Reactivity Rat, Ar Ham, Syr Ham - 1 mL
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogène
Pooled Rat IgG
Clone
RG7-7-6
Isotype
IgG2a kappa
Indications d'application
Optimal working dilution should be determined by the investigator.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C.
Butcher: "A list of monoclonal antibodies specific for alloantigens of the rat." dans: Journal of immunogenetics, Vol. 14, Issue 2-3, pp. 163-76, (1988) (PubMed).
Springer, Bhattacharya, Cardoza, Sanchez-Madrid: "Monoclonal antibodies specific for rat IgG1, IgG2a, and IgG2b subclasses, and kappa chain monotypic and allotypic determinants: reagents for use with rat monoclonal antibodies." dans: Hybridoma, Vol. 1, Issue 3, pp. 257-73, (1984) (PubMed).