Precipitating polyclonal Rabbit antiSerum to Hamster IgG, heavy and light chains.
Réactivité croisée (Details)
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been tested in double radial immunodiffusion with the following results: Bovine ± Cat - Chicken - Dog - Duck - ferret ± Goat - Guinea Pig + Horse + Human - Mouse + Monkey - pigeon - Rat + Sheep - Swine ± Turkey -
Attributs du produit
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in hamster serum, as tested by immunoelectrophoresis and double radial immunodiffusion In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal hamster serum, to prepare an immunoadsorbent for the production of Ig-free hamster serum, plasma or non-Ig preparations.
Purification
Delipidated, heat inactivated, stable whole serum
Stérilité
Heat-inactivated
Immunogène
Purified normal IgG isolated from pooled hamster serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Indications d'application
In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
It is reconstituted by adding 1 mL sterile distilled water.Delipidated, heat inactivated lyophilized stable whole serum. No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum. No foreign proteins added. It is reconstituted by adding 1 mL sterile distilled water.
Buffer
Delipidated, heat inactivated lyophilized stable whole serum. No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum.
Agent conservateur
Without preservative
Stock
RT,4 °C,-20 °C
Stockage commentaire
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product