Lapin anti-Souris IgG1 Anticorps (TRITC)

N° du produit ABIN458467

Détail du produit

Antigène: IgG1

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: TRITC

Application: Immunofluorescence (IF)

Specificité: The reactivity of the antiserum is directed to IgG1 as tested at the level of sensitivity of direct and indirect immunofluorescence staining methods applied to lymphoid cells.

Attributs du produit: Rhodamine isothiocyanate-conjugated IgG fraction of polyclonal rabbit antiserum to mouse IgG1, subclass specific
Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.1.

Purification: Undesired traces of antibody activity are eliminated by immunoaffinity chromatography as required. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogène: Pools of highly purified homogenous IgG1 isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: To identify and measure IgG1, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen or immune complex using a reference antibody of mouse origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Concentration: 10 mg/mL

Buffer: Fluorochrome-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Agent conservateur: Without preservative

Conseil sur la manipulation: Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Détail du antigène

Antigène: IgG1

Abstract: IgG1 Produits

Classe de substances: Antibody