Lapin anti-Mouton IgM (Heavy & Light Chain) Anticorps
WB, IF, ICC, DB, EIA
Polyclonal
IgG
unconjugated
-
- Antigène Tous les produits IgM
- IgM
-
Épitope
- Heavy & Light Chain
-
Reactivité
- Mouton
-
Hôte
- Lapin
-
Clonalité
- Polyclonal
-
Application
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Dot Blot (DB), Enzyme Immunoassay (EIA)
- Purification
- Hyperimmune antisera with strong activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
- Immunogène
- Purified normal IgM isolated from pooled sheep serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
-
-
- Indications d'application
- ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemistry Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Precipitin titre : 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
- Restrictions
- For Research Use only
-
- Buffer
- PBS, pH 7.2 Preservatives: None, as it may interfere with the antibody activity. Stabilizers: None
- Agent conservateur
- Azide free
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
- Date de péremption
- 12 months
-
- Antigène
- IgM
- Abstract
- IgM Produits
- Classe de substances
- Antibody
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