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Lapin anti-Souris Immunoglobulin kappa Chain Complex (Igk) (Light Chain) Anticorps (HRP) - Preadsorbed

Igk ELISA, WB, IHC Polyclonal IgG HRP
N° du produit ABIN5608148
  • Antigène Tous les produits Igk
    Igk (Immunoglobulin kappa Chain Complex (Igk))
    Épitope
    • 20
    • 9
    Light Chain
    Reactivité
    • 33
    • 15
    • 4
    Souris
    Hôte
    • 33
    • 9
    • 6
    • 4
    Lapin
    Clonalité
    • 39
    • 13
    Polyclonal
    Conjugué
    • 10
    • 10
    • 9
    • 7
    • 4
    • 4
    • 4
    • 3
    • 1
    HRP
    Application
    • 42
    • 37
    • 14
    • 14
    • 9
    • 9
    ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
     Réactivité croisée
    Souris
    Attributs du produit
    Anti-Mouse kappa light chain antibody generated in rabbit detects specifically Mouse kappa light chain. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    Purification
    Preadsorption: Solid phase absorption
    Immunogène

    Immunogen: Anti-Mouse kappa light chain was produced by repeated immunization with Mouse kappa light chain in rabbit.

    Immunogen Type: Native Protein

    Isotype
    IgG
  • Indications d'application

    Immunohistochemistry Dilution: 1:500 - 1:2,500

    Application Note: Anti-Mouse kappa light chain antibody generated in rabbit detects specifically Mouse kappa light chain. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

    ELISA Dilution: 1:10,000 - 50,000

    Western Blot Dilution: 1:2,000 - 1:5,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Thimerosal

    Agent conservateur
    Thimerosal (Merthiolate)
    Précaution d'utilisation
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    RT,4 °C,-20 °C
    Stockage commentaire
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Date de péremption
    12 months
  • Antigène
    Igk (Immunoglobulin kappa Chain Complex (Igk))
    Autre désignation
    kappa ) (Igk Produits)
    Sujet

    Synonyms: Rabbit Anti-Mouse κ (kappa chain) Antibody peroxidase Conjugated, Rabbit Anti-Mouse kappa Antibody HRP Conjugation

    Background: Anti-Mouse κ (kappa chain) (RABBIT) Antibody generated in rabbit detects specifically Mouse kappa light chain. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). The light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG kappa is conjugated to peroxidase.

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