Chèvre anti-Souris IgM (Heavy & Light Chain) Anticorps (Alkaline Phosphatase (AP)) - Preadsorbed

N° du produit ABIN965173

Détail du produit

Antigène: IgM

Fragment: F(ab')2 fragment

Épitope: Heavy & Light Chain

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Mouse IgM and Mouse Serum.

Purification: Preadsorption: Solid phase absorption

Stérilité: Sterile filtered

Immunogène:

Immunogen: Mouse IgM whole molecule

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: F(ab')2 Anti-Mouse IgM (mu chain) Antibody has been assayed against 1.0 μg of Mouse IgM in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for F(ab')2 Anti-Mouse IgM (mu chain) Antibody.

ELISA Dilution: 1:5,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.1 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Stock: 4 °C

Stockage commentaire: Store vial at 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: IgM

Abstract: IgM Produits

Classe de substances: Antibody

Sujet:

Synonyms: Goat F(ab')2 Anti-Mouse IgM (mu chain) Antibody Alkaline Phosphatase Conjugation, Goat Fab2 Anti-Mouse IgM mu chain alk phos Conjugated Antibody

Background: F(ab')2 Anti-Mouse IgM (mu chain) Antibody generated in Goat detects specifically Mouse IgM mu heavy chain. F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.