anti-Humain IFT88 anticorps pour Flow Cytometry

Recommended IFT88 Antibody (fourni par: Connectez-vous pour afficher )

Antigène
Intraflagellar Transport 88 Homolog (Chlamydomonas) (IFT88) Anticorps
  • 146590_s_at
  • CG12548
  • CG14465
  • CG15190
  • CG4137
  • Dmel\\CG12548
  • IFT88
  • NOMPB
  • OSM-5
  • Tg737
  • nomp
  • osm-5
  • AW552028
  • Tg737Rpw
  • TgN737Rpw
  • Ttc10
  • flexo
  • fxo
  • orpk
  • polaris
  • D13S1056E
  • DAF19
  • TG737
  • TTC10
  • hTg737
  • intraflagellar transport 88
  • intraflagellar transport 88 homolog
  • intraflagellar transport protein 88 homolog
  • no mechanoreceptor potential B
  • IFT88
  • ift88
  • LOC100518926
  • LOC100640298
  • LOC100646898
  • nompB
  • Ift88
Épitope
C-Term
60
36
12
9
2
2
1
1
1
Reactivité
Humain, Souris
98
74
15
11
5
4
3
2
2
1
1
1
1
Hôte
Chèvre
79
17
3
Clonalité
Polyclonal
Conjugué
Cet anticorp IFT88 est non-conjugé
5
5
5
5
5
5
2
2
2
2
2
2
2
2
2
2
2
Application
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
81
55
50
39
25
22
11
8
2
Options
Fournisseur
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N° du produit (Fournisseur)
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N° du produit ABIN185555
$ 429.84
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General

Antigène Intraflagellar Transport 88 Homolog (Chlamydomonas) (IFT88) Anticorps
Épitope C-Term
(60), (36), (12), (9), (2), (2), (1), (1), (1)
Reactivité Humain, Souris
(98), (74), (15), (11), (5), (4), (3), (2), (2), (1), (1), (1), (1)
Hôte Chèvre
(79), (17), (3)
Clonalité
Polyclonal
Conjugué Cet anticorp IFT88 est non-conjugé
(5), (5), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(81), (55), (50), (39), (25), (22), (11), (8), (2)
Pubmed 3 références disponible
Fournisseur Connectez-vous pour afficher

Détail du produit anti-IFT88 anticorps

Détail du antigène IFT88 Information d'application Stockage References for anti-IFT88 antibody (ABIN185555) Images
Fonction IFT88 / Polaris
Blocking Peptide Blocking peptide for this product available: ABIN369298
Séquence KKRIDEDDFA DEE
Specificité This antibody is expected to recognise many reported isoforms.
Homologie Human, Mouse, Rat, Dog
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Classe de qualité Verified
Immunogène Peptide with sequence C-KKRIDEDDFADEE, from the C Terminus of the protein sequence according to NP_783195.2, NP_006522.2.
Plasmids, Primers & others

Détail du antigène IFT88

Détail du produit anti-IFT88 anticorps Information d'application Stockage References for anti-IFT88 antibody (ABIN185555) Images Haut de la page
Antigène
Autre désignation IFT88 (IFT88 Antibody Extrait)
Sujet IFT88, polaris, TTC10, tetratricopeptide repeat domain 10, HGNC:20606, D13S1056E, DAF19, IFT88, MGC26259, RP11-172H24.2, TG737, hTg737, TPR repeat protein 10, Probe hTg737 (polycystic kidney disease, autosomal recessive), Tg737 protein, intraflagellar transport 88 homolog, recessive polycystic kidney disease protein Tg737 homolog, intraflagellar transport 88 homolog (Chlamydomonas)
ID gène 8100
NCBI Accession NP_783195, NP_006522, NP_001340507, NP_001340498
Pathways Signalisation Hedgehog, Sensory Perception of Sound

Information d'application

Détail du produit anti-IFT88 anticorps Détail du antigène IFT88 Stockage References for anti-IFT88 antibody (ABIN185555) Images Haut de la page
Indications d'application

DS WB Results: Approx 85-90 kDa band observed in lysates of cell lines HepG2 and NIH3T3 and in Human Kidney lysates (calculated MW of 85.3 kDa according to Human NP_001340507.1 and 90.4 kDa according to NP_001340498.1, and 93.1 kDa according to Mouse NP_033402.2 93. and 89.2 kDa according to XP_006518873.1). Recommended concentration: 0.3-1 μg/ul. Primary incubation 1 hour at room temperature.

DS IHC Results: In paraffin embedded Human Kidney shows staining of the brush border in PCT. Recommended concentration: 3 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires

Immunofluorescence: Strong expression of the protein seen within the cytoplasm of HepG2 cells. Recommended concentration: 10μg/ml. This antibody has been successfully used in IF on Mouse Olfactory and Respiratory Epithelium cells as described in the following paper: Miyoshi et al, FASEB J. 2009 Oct,23(10):3289-97, PMID: 19470799.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Restrictions For Research Use only

Stockage

Détail du produit anti-IFT88 anticorps Détail du antigène IFT88 Information d'application References for anti-IFT88 antibody (ABIN185555) Images Haut de la page
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Minimize freezing and thawing.
Stock -20 °C
Stockage commentaire Aliquot and store at -20 °C.

References for anti-IFT88 antibody (ABIN185555)

Détail du produit anti-IFT88 anticorps Détail du antigène IFT88 Information d'application Stockage Images Haut de la page
Produit citée dans:

Miyoshi, Kasahara, Miyazaki, Shimizu, Taniguchi, Matsuzaki, Tohyama, Asanuma: "Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 23, Issue 10, pp. 3289-97, 2009

Jiang, Chiou, Wang, Chien, Ho, Tsai, Lin, Tsai, Li: "Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse." dans: Human molecular genetics, Vol. 18, Issue 9, pp. 1566-77, 2009

Background publications

Vogel: "News focus: Betting on cilia." dans: Science (New York, N.Y.), Vol. 310, Issue 5746, pp. 216-8, 2005

Images

Détail du produit anti-IFT88 anticorps Détail du antigène IFT88 Information d'application Stockage References for anti-IFT88 antibody (ABIN185555) Haut de la page
Images (Fournisseur)
Immunohistochemistry (IHC) image for anti-Intraflagellar Transport 88 Homolog (Chlamydomonas) (IFT88) (C-Term) antibody (ABIN185555) ABIN185555 (3µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen re...