E-cadherin anticorps
Aperçu rapide pour E-cadherin anticorps (ABIN265391)
Antigène
Voir toutes E-cadherin (CDH1) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- This antibody detects endogenous levels of CD324 / Cadherin-1 protein (region surrounding Gly2).
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Réactivité croisée (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
Purification
- Affinity chromatography using epitope-specific immunogen.
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Indications d'application
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ELISA: 1/40000approx. 1/60000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50-1/200. Immunofluorescence: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
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Autre désignation
- CD324 / Cadherin-1
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Sujet
- Cadherins comprise a family of Ca+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxyterminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as beta-Catenin, to regulate cadherin function. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin-6), R-cadherin, B-cadherin, E/P- cadherin and cadherin-5.Synonyms: CAM 120/80, CDH1, CDHE, E-cadherin, Epithelial cadherin, UVO, Uvomorulin
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Poids moléculaire
- approx. 98, 130 kDa
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ID gène
- 999
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NCBI Accession
- NP_004351
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UniProt
- P12830
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Pathways
- Signalisation WNT, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation
Antigène
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