HSP27 anticorps
Aperçu rapide pour HSP27 anticorps (ABIN127002)
Antigène
Voir toutes HSP27 (HSPB1) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- The antibody detects a 27 kDa protein, corresponding to the apparent molecular mass of Hsp27 on SDS-PAGE immunoblots.
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Purification
- Purified
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anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 101-205) antibody
HSPB1 Reactivité: Humain, Souris, Rat WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 Reactivité: Humain, Rat WB, IHC, ELISA, FACS, ICC Hôte: Souris Monoclonal 5D7 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 Reactivité: Humain WB, IHC, IF, FACS, IP, ICC Hôte: Souris Monoclonal 8A7 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 96-205) antibodyHSPB1 Reactivité: Humain WB, ELISA, IF, IP, PLA Hôte: Souris Monoclonal 3G3 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibodyHSPB1 Reactivité: Humain, Souris, Rat WB, IF, FACS, IP, ICC, IHC (p) Hôte: Lapin Polyclonal unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 Reactivité: Humain WB, IHC, ELISA, IF, FACS, IP Hôte: Lapin Monoclonal 10C11 unconjugated Recombinant Antibody
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 Reactivité: Humain, Souris, Rat, Mouton, Singe, Poulet, Chimpanzé WB, IHC, IF, FACS Hôte: Souris Monoclonal G3-1 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (Biotin)HSPB1 Reactivité: Humain WB, IHC, IF, FACS, IP, ICC Hôte: Souris Monoclonal 8A7 Biotin
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (HRP)HSPB1 Reactivité: Humain WB, IHC, IF, FACS, IP, ICC Hôte: Souris Monoclonal 8A7 HRP
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (Atto 390)HSPB1 Reactivité: Humain WB, IHC, IF, FACS, IP, ICC Hôte: Souris Monoclonal 8A7 Atto 390
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Indications d'application
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Western Blot: 1-2 μg/mL. Immunoprecipitation: 5-10 μg/mL. Imunocytochemistry: 10-15 μg/mL. ELISA: 1.0 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 0.2 mg/mL
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Buffer
- PBS, pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal
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Agent conservateur
- Thimerosal (Merthiolate)
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Précaution d'utilisation
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- -20 °C/-80 °C
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Stockage commentaire
- Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.
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- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
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Autre désignation
- HSPB1 / HSP27
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Sujet
- Human Hsp27, mouse Hsp25 and αβ-crystallin are part of a diverse family of small heat shock proteins which are produced in all organisms. They function as chaperone-like proteins by binding unfolded polypeptides and preventing uncontrolled protein aggregation. Hsp27 is believed to exist mainly as oligomers of as many as 8-40 Hsp27 protein monomers in cells and data suggests that the large oligomers of Hsp27 have a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and Hsp70-dependent refolding can occur. Hsp27 is believed to protect cells by enhancing cellular glutathione levels and elevated glutathione levels have been measured in cells overexpressing hsp27. Data from studies using wild-type Hsp27 and mutant forms in which the serine phosphorylation sites were mutated to alanines, glycines or aspartates, have shown that cellular glutathione levels depend on the oligomerization of Hsp27. Recent findings indicate that Hsp27 is also a negative regulator of cytochrome c-dependent activation of procaspase-3.Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27
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ID gène
- 3315
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NCBI Accession
- NP_001531
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UniProt
- P04792
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Pathways
- Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
Antigène
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