MDM2 anticorps (AA 175-200)
Aperçu rapide pour MDM2 anticorps (AA 175-200) (ABIN129683)
Antigène
Voir toutes MDM2 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- AA 175-200
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N° du produit (Fournisseur)
- 600-401-927
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Fournisseur
- Rockland
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Fonction
- Mdm2 Antibody
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Réactivité croisée (Details)
- This affinity-purified antibody is directed against mouse MDM2 protein.
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Attributs du produit
- Synonyms: rabbit anti-MDM2 antibody, MDM-2, E3 ubiquitin-protein ligase Mdm2, p53-binding protein Mdm2, Oncoprotein Mdm2, Double minute 2 protein, RING-type E3 ubiquitin transferase Mdm2, Double minute 2 protein, Hdm2
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Purification
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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Stérilité
- Sterile filtered
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Immunogène
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 175-200 of mouse MDM2.
Immunogen Type: Conjugated Peptide
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Isotype
- IgG
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anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 101-200) antibody
MDM2 Reactivité: Humain WB, ELISA, IHC (p), PLA, RNAi Hôte: Souris Monoclonal 1A7 unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibodyMDM2 Reactivité: Humain, Souris WB, IF, IHC (p), IP, ICC, ChIP Hôte: Lapin Polyclonal unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 130-230) antibodyMDM2 Reactivité: Humain WB, IHC, IF Hôte: Lapin Polyclonal unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 141-176) antibodyMDM2 Reactivité: Humain WB, IF, IHC (p), FACS Hôte: Lapin Polyclonal RB7981 unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (pSer166) antibodyMDM2 Reactivité: Humain, Souris, Singe WB, IHC, ELISA, IF, ICC Hôte: Lapin Polyclonal unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 26-169) antibodyMDM2 Reactivité: Humain IHC, ELISA, FACS Hôte: Souris Monoclonal 3C1E3 unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 393-424) antibodyMDM2 Reactivité: Humain, Souris WB, IHC, ELISA Hôte: Lapin Polyclonal unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (pSer186) antibodyMDM2 Reactivité: Humain WB, ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 200-430) antibodyMDM2 Reactivité: Humain WB, IF Hôte: Lapin Polyclonal unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 119-438) antibodyMDM2 Reactivité: Humain WB, IHC, IHC (p) Hôte: Souris Monoclonal 1E6 unconjugated
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Indications d'application
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Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 90 kDa in size corresponding to MDM2 protein by western blotting in the appropriate cell lysate or extract. See Saucedo, et al (1999) for a discussion on expected molecular weights.
Western Blot Dilution: 1:500 - 1:2,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:3,000 - 1:12,000
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.3 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Date de péremption
- 12 months
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- MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))
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Autre désignation
- Mdm2
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Sujet
- Background: MDM2 is a nuclear phosphoprotein with an apparent molecular mass of 90 kD that forms a complex with the p53 tumor suppressor protein. Human MDM2 was identified as a homologous product of the 'murine double minute 2' gene (mdm2). The MDM2 gene enhances the tumorigenic potential of cells when it is overexpressed and encodes a putative transcription factor. Forming a tight complex with the p53 gene, the MDM2 oncogene can inhibit p53-mediated transactivation. MDM2 binds to p53 and amplification of MDM2 in sarcomas leads to escape from p53-regulated growth control. This mechanism of tumorigenesis parallels that for virus-induced tumors in which viral oncogene products bind to and functionally inactivate p53.
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ID gène
- 17246, 2851543
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UniProt
- P23804
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Pathways
- Signalisation p53, Signalisation PI3K-Akt, Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway
Antigène
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