This IgG fraction antibody of anti-Human IL-1 Receptor Antagonist has been tested for usein Neutralizations, ELISA, Radioimmunoassays, Western blotting, Immunohistochemistryand Flow cytometry. Reactivity in other immunoassays is unknown. Note: This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L]and TMB as a substrate. A working dilution range of 1: 400 to 1: 800 is suggested for thisapplication. This product has been assayed by ELISA against IL-1Receptor Antagonist usingHRP Conjugated Anti-Rabbit IgG [H&L] (Goat) and ABTS as a substrate for 30 minutes atroom temperature. A working dilution range of 1: 500 to 1: 1,000 is suggested for thisproduct. For use in ELISA formats, this antibody is best used as the second antibody incombination with a monoclonal antibody as a capture antibody. This product has beenassayed by Immunohistochemistry. A dilution of 1: 400 or greater is suggested for thisimmunoassay. Either paraffin fixation or cryofixation can be used forImmunohistochemistry. This product has been assayed by radioimmunoassay againstantigen. A dilution of 1: 8,000 is suggested for this immunoassay. Recommended Dilutions: ELISA: 1/1,000-1/5,000. Western Blot: 1/500-1/2,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Stock
4 °C/-20 °C
Stockage commentaire
Store lyophilized product at 2-8 °C. After reconstitution, store at -20 °C.
IL1RA is a member of the Interleukin 1 cytokine family. This protein inhibits the activities of Interleukin 1, alpha (IL1A) and Interleukin 1, beta (IL1B), and modulates a variety of Interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.Synonyms: ICIL-1RA, IL-1RN, IL-1ra, IL1 inhibitor, IL1F3, IRAP, Interleukin-1 receptor antagonist protein