EGF anticorps (AA 45-275) (Biotin)
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- Antigène Voir toutes EGF Anticorps
- EGF (Epidermal Growth Factor (EGF))
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Épitope
- AA 45-275
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Reactivité
- Porc
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp EGF est conjugé à/à la Biotin
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Séquence
- MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- APFLIF SHGNSIFRID LEGTNHEQLV ADAGISVLMD FHYNEERIYW VDLERQLLQR VFLNGTRQEK VCNLEKNVSG MAINWINEEL IWSNQQEGTI TVTDMKGNNS RVLLSALKYP ANVAVDPVER LMFWSSVVAG SLHRADVTGV EVRLLLETSE EIAAVSLDVL DKRLFWIQYN REGGSSRICS CDYDGGSVHF SKHLTQHNVF AMSLFGDHIF YSTWKKKTIW VANKH
- Specificité
- It has been selected for its ability to recognize EGF in immunohistochemical staining and Western blotting.
- Purification
- Affinity Chromatography
- Immunogène
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Recombinant EGF expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against EGF conjugated to biotin. - Isotype
- IgG
- Top Product
- Discover our top product EGF Anticorps primaire
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- Indications d'application
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Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - Commentaires
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Content: The quality control contains recombinant EGF (Ala45~His275) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % . - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Conseil sur la manipulation
- Avoid repeated freeze/thaw cycles
- Stock
- 4 °C
- Stockage commentaire
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Date de péremption
- 12 months
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- Antigène
- EGF (Epidermal Growth Factor (EGF))
- Abstract
- EGF Produits
- Synonymes
- anticorps HOMG4, anticorps URG, anticorps AI790464, anticorps CEGF, anticorps epidermal growth factor, anticorps pro-epidermal growth factor, anticorps EGF, anticorps egf, anticorps CpipJ_CPIJ020278, anticorps Egf
- Pathways
- Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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