FYN anticorps (AA 7-176)

N° du produit ABIN94311

Cité dans 2 publications. Cet anticorps anti-FYN Monoclonal Souris (Clone FYN-01) (ABIN94311) détecte spécifiquement FYN dans WB, IP, ICC et IHC (p). L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour FYN anticorps (AA 7-176) (ABIN94311)

Antigène: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FYN est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: FYN-01

Détail du produit anti-FYN anticorps

Épitope: AA 7-176

Fonction: Anti-FYN Purified

Réactivité croisée (Details): Mouse, Human, Other not tested

Attributs du produit: The mouse monoclonal antibody FYN-01 recognizes Fyn, a 59 kDa non-receptor Src-family protein tyrosine kinase (intracellular antigen).

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Bacterially expressed recombinant fragment of human Fyn (aa 7-176).

Isotype: IgG2b

Information d'application

Indications d'application: Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL, positive tissue: brain.
Western blotting: Recommended dilution: 2 μg/mL, positive control: JURKAT human T cell leukemia cell line.
Immunoprecipitation: positive control: J77 T cell line. Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1 % laurylmaltosid (w/v), 50 mM NaF). The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-FYN (ABIN94311)

Queval, Nicolas, Beau: "Role of Src kinases in mobilization of glycosylphosphatidylinositol-anchored decay-accelerating factor by Dr fimbria-positive adhering bacteria." dans: Infection and immunity, Vol. 79, Issue 7, pp. 2519-34, (2011) (PubMed).

Meraner, Horejsí, Wolpl, Fischer, Stingl, Maurer: "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 4, pp. 2262-71, (2007) (PubMed).

Détails sur FYN

Antigène: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Autre désignation: FYN

Sujet: FYN proto-oncogene, Src family tyrosine kinase,Fyn is a ubiquitously expressed Src-family protein tyrosine kinase with important roles e.g. in immune and nervous system. It regulates N-methyl-D-aspartate (NMDA) receptor functions, thus affecting various brain functions, and even many of its other substrates are important for neural migration, synaptic plasticity, oligodendrocyte differentiation, and axon growth and guidance. In immune system Fyn namely regulates the commitment of T cells to activation, is important in T cell anergy induction, promotes mast cell chemotaxis and reorganization of cytoskeleton and participates in mast cell activation. Fyn is also involved in embryonic stem cell growth and differentiation, associates with tubulin and may play roles in mitotic spindle formation.,p59Fyn, SLK, SYN

ID gène: 2534

UniProt: P06241

Pathways: Signalistation JAK/STAT, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling