anti-Rat (Rattus) CREB1 anticorps pour Western Blotting

Recommended CREB1 Antibody (fourni par: Connectez-vous pour afficher )

Antigène
CAMP Responsive Element Binding Protein 1 (CREB1) Anticorps
  • creb
  • CREB1
  • Creb1
  • CREB
  • 2310001E10Rik
  • 3526402H21Rik
  • AV083133
  • Creb
  • Creb-1
  • creb1
  • zgc:55598
  • cAMP responsive element binding protein 1
  • cAMP responsive element binding protein 1a
  • cyclic AMP response element binding protein
  • CREB1
  • creb1
  • Creb1
  • LOC100351999
  • creb1a
  • CREB
Reactivité
Humain, Souris, Rat (Rattus), Singe
548
328
298
41
38
36
30
26
26
26
19
17
15
12
7
7
7
2
1
1
1
1
Hôte
Souris
450
100
11
1
Clonalité
Monoclonal
Conjugué
Cet anticorp CREB1 est non-conjugé
18
15
15
7
7
6
6
6
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
441
224
191
100
92
48
43
42
31
11
11
10
4
3
3
2
2
1
1
1
Options
Fournisseur
Connectez-vous pour afficher
Supplier Product No.
Connectez-vous pour afficher

Images

N° du produit ABIN969061
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
3.1424623 ABIN2780104 WB Rabbit N-Term Connectez-vous pour afficher Polyclonal 1
3.1424623 ABIN2778454 WB Rabbit Middle Region Connectez-vous pour afficher Polyclonal 1
3.1424623 ABIN2777244 WB Rabbit N-Term Connectez-vous pour afficher Polyclonal 1
3.1424623 ABIN2778453 WB Rabbit N-Term Connectez-vous pour afficher Polyclonal
3.1424623 ABIN2792114 WB Rabbit N-Term Connectez-vous pour afficher Polyclonal 1
3.1424623 ABIN2705936 IHC WB Rabbit pSer121 Connectez-vous pour afficher Polyclonal
3.1424623 ABIN2705937 IP IHC WB Rabbit pSer133 Connectez-vous pour afficher Polyclonal
3.1424623 ABIN2705940 IC IF IHC WB Rabbit Center Connectez-vous pour afficher Polyclonal
3.1424623 ABIN2705938 IC IF IHC WB Rabbit Center Connectez-vous pour afficher Polyclonal
3.1424623 ABIN2705939 IHC WB Rabbit Center Connectez-vous pour afficher Polyclonal
3.1424623 ABIN3042756 WB Rabbit IgG AA 1-177 Connectez-vous pour afficher Polyclonal
3.1424623 ABIN1531812 ELISA IHC IP WB Rabbit IgG AA 100-149, pSer133 Connectez-vous pour afficher Polyclonal
3.1424623 ABIN1532006 ELISA WB Rabbit IgG AA 71-120, pThr100 Connectez-vous pour afficher Polyclonal
3.1424623 ABIN1531175 IF IHC ELISA WB Rabbit IgG AA 111-160, pSer142 Connectez-vous pour afficher Polyclonal
3.1424623 ABIN1532192 IF IHC ELISA WB Rabbit IgG AA 111-160 Connectez-vous pour afficher Polyclonal
3.1424623 ABIN1532330 IHC ELISA WB Rabbit IgG AA 91-140 Connectez-vous pour afficher Polyclonal
3.1424623 ABIN1532841 IHC ELISA WB Rabbit IgG AA 96-145 Connectez-vous pour afficher Polyclonal
3.1424623 ABIN1531311 ELISA WB Rabbit IgG AA 91-140, pSer121 Connectez-vous pour afficher Polyclonal
3.1424623 ABIN1533043 ELISA WB Rabbit IgG AA 71-120 Connectez-vous pour afficher Polyclonal
3.1424623 ABIN1532842 IHC ELISA WB Rabbit IgG AA 100-149 Connectez-vous pour afficher Polyclonal

General

Antigène CAMP Responsive Element Binding Protein 1 (CREB1) Anticorps
Reactivité Humain, Souris, Rat (Rattus), Singe
(548), (328), (298), (41), (38), (36), (30), (26), (26), (26), (19), (17), (15), (12), (7), (7), (7), (2), (1), (1), (1), (1)
Hôte Souris
(450), (100), (11), (1)
Clonalité (Clone)
Monoclonal   ( )
Conjugué Cet anticorp CREB1 est non-conjugé
(18), (15), (15), (7), (7), (6), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(441), (224), (191), (100), (92), (48), (43), (42), (31), (11), (11), (10), (4), (3), (3), (2), (2), (1), (1), (1)
Pubmed 3 références disponible
Fournisseur Connectez-vous pour afficher

Détail du produit anti-CREB1 anticorps

Détail du antigène CREB1 Information d'application Stockage References for anti-CREB1 antibody (ABIN969061) Images
Purification Ascites
Immunogène Purified recombinant fragment of human CREB1 expressed in E. Coli.
Clone 5G3
Isotype IgG1

Détail du antigène CREB1

Détail du produit anti-CREB1 anticorps Information d'application Stockage References for anti-CREB1 antibody (ABIN969061) Images Haut de la page
Antigène
Autre désignation CREB1 (CREB1 Antibody Extrait)
Sujet This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. (provided by RefSeq)
Poids moléculaire 43 kDa
ID gène 1385
HGNC 1385
Pathways Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation

Information d'application

Détail du produit anti-CREB1 anticorps Détail du antigène CREB1 Stockage References for anti-CREB1 antibody (ABIN969061) Images Haut de la page
Indications d'application ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000
Restrictions For Research Use only

Stockage

Détail du produit anti-CREB1 anticorps Détail du antigène CREB1 Information d'application References for anti-CREB1 antibody (ABIN969061) Images Haut de la page
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C,-20 °C
Stockage commentaire 4°C, -20°C for long term storage

References for anti-CREB1 antibody (ABIN969061)

Détail du produit anti-CREB1 anticorps Détail du antigène CREB1 Information d'application Stockage Images Haut de la page
Produit citée dans:

Jansson, Ng, Fu, Depatie, Al Azzabi, Screaton: "Glucose controls CREB activity in islet cells via regulated phosphorylation of TORC2." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 29, pp. 10161-6, 2008

Mamdani, Alda, Grof, Young, Rouleau, Turecki: "Lithium response and genetic variation in the CREB family of genes." dans: American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, Vol. 147B, Issue 4, pp. 500-4, 2008

Seo, Chung: "CREB activates proteasomal degradation of DSCR1/RCAN1." dans: FEBS letters, Vol. 582, Issue 13, pp. 1889-93, 2008

Images

Détail du produit anti-CREB1 anticorps Détail du antigène CREB1 Information d'application Stockage References for anti-CREB1 antibody (ABIN969061) Haut de la page
Supplier Images
ELISA image for anti-CAMP Responsive Element Binding Protein 1 (CREB1) antibody (ABIN969061) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Immunocytochemistry (ICC) image for anti-CAMP Responsive Element Binding Protein 1 (CREB1) antibody (ABIN969061) Figure 2: Immunofluorescence analysis of Hela cells using CREB1 mouse mAb (green) . R...
Immunohistochemistry (IHC) image for anti-CAMP Responsive Element Binding Protein 1 (CREB1) antibody (ABIN969061) Figure 3: Immunohistochemical analysis of paraffin-embedded prostate cancer tissues (...
Western Blotting (WB) image for anti-CAMP Responsive Element Binding Protein 1 (CREB1) antibody (ABIN969061) Figure 4: Western blot analysis using CREB1 mouse mAb against K562 (1), Jurkat (2), L...
'Independent Validation' signe
Antigène CREB1
Numéro du lot 110517
Application validée Western Blotting
Contrôle positif primary mouse microglia
Contrôle négative siRNA knock-down of CREB1 in primary mouse microglia
Conclusion Passed. ABIN969061 specifically recognizes Creb1 in primary mouse microglia lysates.
Anticorps primaire ABIN969061
Anticorps secondaire anti-mouse HRP-conjugated antibody (DakoCytomation, P0447, lot 00058737)
Protocole
  • Primary mouse microglia are grown in DMEM (Invitrogen), supplemented with 10% foetal bovine serum (Gibco), 10% horse serum and penicillin and streptomycin (Gibco), at 37°C and 5% CO2 in a 12-well dish.
  • Transfect cells with 0.05nmol mouse Creb1 Silencer Select siRNA (ThermoFisher Scientific, ASO1093M, ASO10982 and ASO109CG, lot AMO04BXD) using Lipofectamine 3000 (Invitrogen) following the manufacturer’s instructions.
  • Lyse cells in 25µl per well cold lysis buffer (10mM Tris-HCl pH 8.0, 150mM NaCl, 1% Nonidet-P40, 1mM EDTA, 10mM NaF, 1mM Na3VO4, containing protease inhibitors (Sigma)).
  • Determine total protein content of the lysates using Bradford assay (Bio-Rad, 500-0006, lot 111832).
  • Denature 5µg of total protein for 5min at 95°C in 5µl Laemmli SDS sample buffer and subsequently separate them on a denaturing, freshly cast 10% SDS-PAGE for 1h at 140V.
  • Transfer proteins onto PVDF membrane (Pall Life Sciences, 75696G, lot TO3225) with a semi-dry Western blotting system for 50min at 60mA.
  • Block the membrane with TBST (TBS, 0.1% Tween) containing 5% milk ON at 4°C.
  • Incubation with primary mouse anti-CREB1 antibody (antibodies-online, ABIN969061, lot 110517) diluted 1:10000 in TBST containing 5% milk for 1h at RT.
  • Wash membrane 5x 5min with TBST.
  • Incubation with anti-mouse horseradish peroxidase-conjugated secondary antibody (DakoCytomation, P0447, lot 00058737) diluted 1:2000 in TBST containing 5% milk for 1h at RT.
  • Wash membrane 5x 5min with TBST and 3x 5min with TBS.
  • Reveal protein bands using self-made ECL Western Blotting Detection Reagent with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
  • Strip membranes using RestoreTM Western Blot Stripping Buffer (Thermo Scientific, 21059, lot NA 164269) and subsequently incubate with a GAPDH loading control antibody (Sigma, G9545, lot 049K4787) diluted 1:10000 and with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000.
  • Wash and reveal the membrane as described above.
Notes The CREB1 antibody ABIN969061 reveals a protein of the expected molecular weight of murine Creb1 in lysates of primary mouse microglia. The protein band’s intensity decreases upon siRNA knockdown.
Validation Images
Western Blotting validation image for anti-CAMP Responsive Element Binding Protein 1 (CREB1) antibody (ABIN969061) Primary mouse microglia were transfected with Creb1 siRNA and Creb1 was revealed usin...