Lapin anti-Boeuf (Vache) IgG (F(ab')2 Region) Anticorps (FITC) - Preadsorbed

Détails pour le produit réf. ABIN100926
Antigène
Épitope
F(ab')2 Region
855
232
202
76
38
13
Reactivité
Boeuf (Vache)
246
226
173
156
135
80
80
57
54
54
47
41
40
38
25
16
14
13
8
7
7
3
2
1
Hôte
Lapin
626
515
205
49
39
36
10
7
5
3
1
Clonalité
Polyclonal
Conjugué
FITC
184
172
158
135
129
128
50
35
32
30
28
25
22
16
11
9
9
9
9
8
8
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
Options
Immunogène

Immunogen: Bovine IgG F(ab')2 fragment

Isotype IgG
Specificité IgG F(ab')2
Attributs du produit Concentration Definition: by UV absorbance at 280 nm
Purification Preadsorption: Solid phase absorption
Labeling Ratio 1.9
Antigène
Sujet

Synonyms: rabbit anti-Bovine IgG F(ab')2 fluorescein Conjugated Antibody, rabbit anti-Bovine IgG Fab2 FITC Conjugated Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.

Indications d'application

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Commentaires

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 2.0 mg/mL
Buffer

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Product is photosensitive and should be protected from light.
Stock RT,4 °C,-20 °C
Date de péremption 12 months
Background publications Blakeslee, Baines: "Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgG." dans: Journal of immunological methods, Vol. 13, Issue 3-4, pp. 305-20, 1977 (PubMed).