HTR7 anticorps (C-Term)

N° du produit ABIN1048279

Détail du produit anti-HTR7 anticorps

Antigène: HTR7 (5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7))

Épitope: C-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HTR7 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Marque: IHC-plus™

Specificité: Human 5HT7 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%) Monkey, Marmoset (94%) Bovine (88%) Mouse, Panda, Dog, Horse (81%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 16 amino acid peptide from C-terminus of human 5HT7 Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%), Monkey, Marmoset (94%), Bovine (88%), Mouse, Panda, Dog, Horse (81%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 - 20 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur HTR7

Antigène: HTR7 (5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7))

Autre désignation: HTR7 / 5-HT7 Receptor (HTR7 Produits)

Synonymes: anticorps 5-HT7, anticorps 5-HT-dro, anticorps 5-HT-dro1, anticorps 5-HT7Dro, anticorps 5-HT[7]Dro, anticorps 5-HT[[7Dro]], anticorps 5-HT[[7]], anticorps 5-HT[[7]]Dro, anticorps 5-HT[[dro1]], anticorps 5-HTdro1, anticorps 5HT-7, anticorps 5HT-R1, anticorps 5HT-dro, anticorps 5HT-dro1, anticorps 5HT[[7Dro]], anticorps 5htdror, anticorps CG12073, anticorps DRO1, anticorps Dm5HTdro1, anticorps Dmel\\CG12073, anticorps 5Ht7, anticorps 5HT7, anticorps htr7-A, anticorps 5-hydroxytryptamine receptor 7, anticorps 5-hydroxytryptamine (serotonin) receptor 7, anticorps 5-hydroxytryptamine (serotonin) receptor 7, adenylate cyclase-coupled, anticorps 5-hydroxytryptamine (serotonin) receptor 7, adenylate cyclase-coupled L homeolog, anticorps HTR7, anticorps 5-HT7, anticorps LOC752003, anticorps Htr7, anticorps htr7.L

Sujet: Name/Gene ID: HTR7
Subfamily: Serotonin
Family: GPCR

Synonyms: HTR7, 5-HT7, 5-HT7 receptor, 5-HT-7, 5-hydroxytryptamine 7 receptor, 5HT7, 5-HT-X, 5-hydroxytryptamine receptor 7, 5HT7 Receptor, HT7, Serotonin 5-HT7 receptor, Serotonin receptor 7

ID gène: 3363

Pathways: Signalistation JAK/STAT