CACNA1C anticorps (Internal Region)

N° du produit ABIN1048367

Détail du produit anti-CACNA1C anticorps

Antigène: CACNA1C (Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C))

Épitope: Internal Region

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CACNA1C est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human CACNA1C / Cav1.2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gibbon (100%) Gorilla, Monkey, Marmoset (94%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 16 amino acid peptide from internal region of human CACNA1C / Cav1.2. Percent identity with other species by BLAST analysis: Human, Gibbon (100%), Gorilla, Monkey, Marmoset (94%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (2.5 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur CACNA1C

Antigène: CACNA1C (Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C))

Autre désignation: CACNA1C / Cav1.2 (CACNA1C Produits)

Synonymes: anticorps CACH2, anticorps CACN2, anticorps CACNL1A1, anticorps CCHL1A1, anticorps CaV1.2, anticorps LQT8, anticorps TS, anticorps Cav1.2, anticorps Cchl1a1, anticorps MBC, anticorps MELC-CC, anticorps RATIVS302, anticorps isl, anticorps C-LTCC, anticorps CHCACHA1C, anticorps ICA, anticorps cav1.2, anticorps ca(v)1.2, anticorps CAV1.2, anticorps BCR, anticorps rbc, anticorps calcium voltage-gated channel subunit alpha1 C, anticorps calcium channel, voltage-dependent, L type, alpha 1C subunit, anticorps voltage-dependent L-type calcium channel subunit alpha-1C, anticorps calcium channel, voltage-dependent, L type, alpha 1C subunit S homeolog, anticorps Red blood cell count, anticorps RNA, 7SL, cytoplasmic 263, pseudogene, anticorps CACNA1C, anticorps Cacna1c, anticorps cacna1c, anticorps LOC100442085, anticorps cacna1c.S, anticorps RBC, anticorps RN7SL263P

Sujet: Name/Gene ID: CACNA1C
Subfamily: Calcium channel - alpha subunit Cav1
Family: Ion Channel

Synonyms: CACNA1C, Alpha 1c, CaV1.2, CACH2, CACNL1A1, CCHL1A1, DHPR, alpha-1 subunit, DHP receptor, DHPr, LQT8, TS, CACN2

ID gène: 775

Pathways: Hormone Transport, Carbohydrate Homeostasis