NR4A1 anticorps (N-Term)
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- Antigène Voir toutes NR4A1 Anticorps
- NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))
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Épitope
- N-Term
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Reactivité
- Humain, Souris, Rat, Hamster
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp NR4A1 est non-conjugé
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human NUR77. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster (100%) Marmoset, Elephant, Panda, Bovine, Dog, Horse (93%) Bat, Rabbit (87%).
- Purification
- Immunoaffinity purified
- Immunogène
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Synthetic 15 amino acid peptide from N-terminal to DNA binding domain of human NUR77. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster (100%), Marmoset, Elephant, Panda, Bovine, Dog, Horse (93%), Bat, Rabbit (87%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product NR4A1 Anticorps primaire
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- Indications d'application
- Approved: IHC, IHC-P (16 μg/mL)
- Commentaires
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Target Species of Antibody: Human
- Procédure de l'essai
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))
- Autre désignation
- NR4A1 / NUR77 (NR4A1 Produits)
- Synonymes
- anticorps GFRP1, anticorps HMR, anticorps N10, anticorps NAK-1, anticorps NGFIB, anticorps NP10, anticorps NUR77, anticorps TR3, anticorps NGFI-B, anticorps Gfrp, anticorps Hbr-1, anticorps Hbr1, anticorps Hmr, anticorps TIS1, anticorps nur77, anticorps Ngfi-b, anticorps Nur77, anticorps nuclear receptor subfamily 4 group A member 1, anticorps nuclear receptor subfamily 4, group A, member 1, anticorps NR4A1, anticorps Nr4a1
- Sujet
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Name/Gene ID: NR4A1
Subfamily: NR4 Nerve growth factor-like
Family: NHR
Synonyms: NR4A1, GFRP1, Early response protein NAK1, HMR, NGFIB, Orphan nuclear receptor TR3, Orphan receptor tr3, Steroid receptor TR3, TR3, NAK1, NGFI-B, NP10, Receptor NGFIB, Testicular receptor 3, TR3 orphan receptor, N10, NAK-1, NUR77, Orphan nuclear receptor HMR - ID gène
- 3164
- Pathways
- Fc-epsilon Receptor Signaling Pathway, Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Steroid Hormone Mediated Signaling Pathway
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