It has been selected for its ability to recognize AP in immunohistochemical staining and Western blotting.
Purification
Affinity Chromatography
Immunogène
Native aprotinin of humanThe aprotinin is the small protein pancreatic trypsin inhibitor, or BPTI, which inhibits trypsin and related proteolytic enzymes. Aprotinin was used as a medication administered by injection to reduce bleeding during complex surgery, such as heart and liver surgery. In cardiac surgery with a high risk of significant blood loss, aprotinin significantly reduced bleeding, mortality and hospital stay. Beneficial effects were also reported in high-risk orthopedic surgery. In liver transplantation, initial reports of benefit were overshadowed by concerns about toxicity. The antibody is a rabbit polyclonal antibody raised against AP.
Aprotinin
Reactivité: Boeuf (Vache)
WB, ELISA, IHC, IC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Conseil sur la manipulation
Avoid repeated freeze/thaw cycles
Stock
4 °C
Stockage commentaire
Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.