ATP7A anticorps (C-Term)

N° du produit ABIN1098198

Détail du produit anti-ATP7A anticorps

Antigène: ATP7A (ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A))

Épitope: AA 42-61, C-Term

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ATP7A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)

Fonction: Anti-Copper-Transporting ATPase1 Mouse Monoclonal Antibody

Specificité: This antibody recognizes human, mouse, and rat Copper-Transporting ATPase1.Immunohistochemistry: use at 1-5 μg/mL. These are recommended concentrations.Enduser should determine optimal concentrations for their

Réactivité croisée: Humain, Souris, Rat

Purification: Purified by Protein G affinity chromatography.

Immunogène: Synthetic peptide correspond- ing to aa 42-61 (cytoplasmic C-terminus) of human Copper-Transporting ATPase1 (accession no. NP_000043.3).

Clone: S60-4

Isotype: IgG2b

Information d'application

Indications d'application: Immunoblotting: use at 1-2 μg/mL. A band of ~180 kDa is detected.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Détails sur ATP7A

Antigène: ATP7A (ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A))

Autre désignation: Copper-Transporting ATPase1 (ATP7A Produits)

Synonymes: anticorps ATP7A, anticorps cal, anticorps wu:fc43e01, anticorps zgc:153422, anticorps zgc:158633, anticorps DDBDRAFT_0218568, anticorps DDBDRAFT_0235190, anticorps DDB_0218568, anticorps DDB_0235190, anticorps atpase, anticorps Atp7a, anticorps kal, anticorps atp7a, anticorps DSMAX, anticorps MK, anticorps MNK, anticorps SMAX3, anticorps Blo, anticorps DXHXS1608e, anticorps I14, anticorps Mo, anticorps blotchy, anticorps br, anticorps brindled, anticorps mottled, anticorps Mnk, anticorps ATPase copper transporting alpha, anticorps ATPase, Cu++ transporting, alpha polypeptide, anticorps P-type ATPase, anticorps ATP synthase subunit a, anticorps copper-transporting ATPase 1, anticorps ATP7A, anticorps atp7a, anticorps LOC100049514, anticorps Atp7a, anticorps LOC412379

Sujet: Copper-transporting ATPase 1,ATP-driven copper (Cu(+)) ion pump that plays an important role in intracellular copper ion homeostasis (PubMed:10419525, PubMed:11092760, PubMed:28389643). Within a catalytic cycle, acquires Cu(+) ion from donor protein on the cytoplasmic side of the membrane and delivers it to acceptor protein on the lumenal side. The transfer of Cu(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing to outward-facing state (PubMed:10419525, PubMed:19453293, PubMed:19917612, PubMed:31283225, PubMed:28389643). Under physiological conditions, at low cytosolic copper concentration, it is localized at the trans-Golgi network (TGN) where it transfers Cu(+) ions to cuproenzymes of the secretory pathway (PubMed:28389643, PubMed:11092760). Upon elevated cytosolic copper concentrations, it relocalizes to the plasma membrane where it is responsible for the export of excess Cu(+) ions (PubMed:10419525, PubMed:28389643). May play a dual role in neuron function and survival by regulating cooper efflux and neuronal transmission at the synapse as well as by supplying Cu(+) ions to enzymes such as PAM, TYR and SOD3 (PubMed:28389643) (By similarity). In the melanosomes of pigmented cells, provides copper cofactor to TYR to form an active TYR holoenzyme for melanin biosynthesis (By similarity). {UniProtKB:Q64430, PubMed:10419525, PubMed:11092760, PubMed:19453293, PubMed:19917612, PubMed:28389643, PubMed:31283225}.,The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from cells. ATP7A (also known as Copper-Transporting ATPase1) functions as a transmembrane copper-translocating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Although ATP7A is not detectable in most normal tissues, it is expressed in many common tumor types. Increased expression of ATP7A renders tumor cells resistant to cisplatin and carboplatin.,Golgi apparatus, trans-Golgi network membrane, Cell membrane, Melanosome membrane, Early endosome membrane, Cell projection, axon, Cell projection, dendrite, Cell junction, synapse, postsynaptic density,EC 7.2.2.8, Copper pump 1, Menkes disease-associated protein

UniProt: Q04656

Pathways: Transition Metal Ion Homeostasis, Ribonucleoside Biosynthetic Process