GABBR1 anticorps (C-Term)

N° du produit ABIN1098201

Détail du produit anti-GABBR1 anticorps

Antigène: GABBR1 (gamma-aminobutyric Acid (GABA) B Receptor, 1 (GABBR1))

Épitope: AA 873-977, C-Term

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GABBR1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC)

Fonction: Anti-GABA (B)R1 Mouse Monoclonal Antibody

Specificité: This antibody recognizes human, mouse, and rat GABA(B)R1. It does not cross- react with GABA(B)R2.

Réactivité croisée: Humain, Souris, Rat

Réactivité croisée (Details): No cross Reactivity with GABA(B)R2

Purification: Purified by Protein G affinity chromatography.

Immunogène: Fusion protein corresponding to aa 873-977 (cytoplasmic C-terminus) of rat GABA(B)R1 (accession no. NP_112290.2).

Clone: S93A-49

Isotype: IgG1

Information d'application

Indications d'application: Immunoblotting: use at 1-2 μg/mL. A band of ~115 kDa is detected.
Immunocytochemistry: use at 1-5 μg/mL.
These are recommended concentrations.
Enduser should determine optimal concentrations for their applications.
Positive control: Rat brain membranes.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Détails sur GABBR1

Antigène: GABBR1 (gamma-aminobutyric Acid (GABA) B Receptor, 1 (GABBR1))

Autre désignation: GABA(B)R1 (GABBR1 Produits)

Synonymes: anticorps GABABR1, anticorps GABBR1-3, anticorps GB1, anticorps GPRC3A, anticorps dJ271M21.1.1, anticorps dJ271M21.1.2, anticorps gabbr1, anticorps GABBR1, anticorps GABA(B)R1, anticorps GABAB1, anticorps GABAbR1, anticorps bM573K1.1, anticorps gamma-aminobutyric acid type B receptor subunit 1, anticorps gamma-aminobutyric acid (GABA) B receptor, 1a, anticorps gamma-aminobutyric acid (GABA) B receptor, 1, anticorps GABBR1, anticorps gabbr1a, anticorps gabbr1, anticorps Gabbr1

Sujet: γ-aminobutyric acid type B receptor subunit 1,Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed:9872315, PubMed:9872317, PubMed:9872744). Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (PubMed:9872317, PubMed:10658574). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed:10075644, PubMed:9872315, PubMed:9872744, PubMed:10924501). Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed:9069281, PubMed:10457184, PubMed:9872315, PubMed:9872744, PubMed:10924501, PubMed:10692480). Calcium is required for high affinity binding to GABA (PubMed:10692480). Plays a critical role in the fine-tuning of inhibitory synaptic transmission (PubMed:9872744). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed:9872744, PubMed:10924501, PubMed:10692480). Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (By similarity). {UniProtKB:Q9UBS5, PubMed:10075644, PubMed:10457184, PubMed:10658574, PubMed:10692480, PubMed:10924501, PubMed:9069281, PubMed:9872315, PubMed:9872744}.,GABA (g-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B), and GABA(C). GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission. Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 in which GABA(B)R1 binds a ligand and GABA(B)R2 is the primary G protein contact site.,Cell membrane, Cell junction, synapse, postsynaptic cell membrane, Cell projection, dendrite, Perikaryon,GABA-B receptor 1, GABA-B-R1, GABA-BR1, GABABR1, Gb1

UniProt: Q9Z0U4

Pathways: Positive Regulation of Peptide Hormone Secretion, cAMP Metabolic Process