beta Actin anticorps (AA 359-368) (Biotin)

N° du produit ABIN1100217

Détail du produit anti-beta Actin anticorps (Biotin)

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Épitope: AA 359-368

Reactivité: Humain, Souris, Rat, Singe, Porc, Mouton, Boeuf (Vache), Hamster, Cobaye, Chévre

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp beta Actin est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificité: A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.

Purification: Immunoaffinity purified

Immunogène: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 359-368 of Human beta Actin.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)

Usage: This affinity-purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kD in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: avoid freeze thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Détails sur beta Actin

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Autre désignation: ACTB / Beta Actin (ACTB Produits)

Synonymes: anticorps BRWS1, anticorps PS1TP5BP1, anticorps ACTA1, anticorps Actx, anticorps E430023M04Rik, anticorps beta-actin, anticorps ps1tp5bp1, anticorps act-2, anticorps actb, anticorps B-actin, anticorps LOC100174883, anticorps LOC100304816, anticorps A, anticorps ACT5C, anticorps Ac5C, anticorps Act, anticorps Act-5C, anticorps Act5, anticorps Act5c, anticorps Actin, anticorps Actin/BAP47, anticorps Actin5C, anticorps BAP47, anticorps Bap47, anticorps CG4027, anticorps Dmel\\CG4027, anticorps M32055, anticorps T11, anticorps act, anticorps act 5C, anticorps act5C, anticorps actin, anticorps actin5C, anticorps anon-EST:fe2D2, anticorps beta-actin/Bap47, anticorps cyt5C, anticorps l(1)G0009, anticorps l(1)G0010, anticorps l(1)G0025, anticorps l(1)G0079, anticorps l(1)G0117, anticorps l(1)G0177, anticorps l(1)G0245, anticorps l(1)G0330, anticorps l(1)G0420, anticorps l(1)G0486, anticorps Bact, anticorps ACT-5, anticorps ACTB, anticorps B-ACTZF, anticorps actba, anticorps bact, anticorps bactin1, anticorps bactzf, anticorps wu:fd18f05, anticorps A26C1B, anticorps POTE2alpha, anticorps POTEACTIN, anticorps Beta-actin, anticorps actin beta, anticorps actin, beta, anticorps cytoplasmic actin OlCA1, anticorps beta-actin, anticorps Actin 5C, anticorps actin gamma 1, anticorps actin, beta 1, anticorps actin, beta L homeolog, anticorps beta actin, anticorps POTE ankyrin domain family member F, anticorps actin, beta like 2, anticorps actin, cytoplasmic 1, anticorps ACTB, anticorps Actb, anticorps actb, anticorps LOC100174883, anticorps LOC100304816, anticorps Act5C, anticorps ACTG1, anticorps actb1, anticorps actb.L, anticorps LOC100136352, anticorps POTEF, anticorps ACTBL2, anticorps LOC109073280

Sujet: Name/Gene ID: ACTB

Synonyms: ACTB, Actin, cytoplasmic 1, Beta-actin, Beta actin, BRWS1, Actin, beta, Beta cytoskeletal actin, PS1TP5-binding protein 1, PS1TP5BP1

ID gène: 60

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction