IL-4 anticorps
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- Antigène Voir toutes IL-4 (IL4) Anticorps
- IL-4 (IL4) (Interleukin 4 (IL4))
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp IL-4 est non-conjugé
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Neutralization (Neut), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human IL-4
- Purification
- Immunoaffinity purified
- Immunogène
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E.coli derived recombinant Human IL-4.
Type of Immunogen: Cell extract - Isotype
- IgG
- Top Product
- Discover our top product IL4 Anticorps primaire
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- Indications d'application
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Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (3 - 5 μg/mL), SwELISA, WB
Usage: Immunohistochemistry: LS-B7474 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-4 (1.50 ng/mL), a concentration of 03-05 μg/mL of this antibody is required. ELISA: To detect hIL-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-4 (LS-C104301) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-4. Western Blot: To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Commentaires
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Conseil sur la manipulation
- Avoid freeze/thaw cycles.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Antigène
- IL-4 (IL4) (Interleukin 4 (IL4))
- Autre désignation
- IL4 (IL4 Produits)
- Synonymes
- anticorps IL4, anticorps IL-4, anticorps BCGF-1, anticorps BCGF1, anticorps BSF-1, anticorps BSF1, anticorps Il-4, anticorps Il4e12, anticorps IL2, anticorps interleukin 4, anticorps IL4, anticorps Il4
- Sujet
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Name/Gene ID: IL4
Family: Hormone
Synonyms: IL4, B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, BCGF1, BSF-1, Binetrakin, BCGF-1, Interleukin 4, Interleukin-4, Pitrakinra, BSF1, IL-4 - ID gène
- 3565
- UniProt
- P05112
- Pathways
- Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
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