Desmoplakin anticorps

N° du produit ABIN111818

L’anticorps anti-Desmoplakin Monoclonal Souris est utilisé pour la détection de Desmoplakin dans des échantillons de Humain, Souris, Rat, Poulet, Boeuf (Vache), Amphibien et Chien. Il a été validé pour WB, IF et IHC (fro).

Aperçu rapide pour Desmoplakin anticorps (ABIN111818)

Antigène: Desmoplakin (DSP)

Reactivité: Humain, Souris, Rat, Poulet, Boeuf (Vache), Amphibien, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Desmoplakin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: DP2-15

Détail du produit anti-Desmoplakin anticorps

Specificité: This antibody reacts to Desmoplakin 1&2. It shows distinct punctate membrane staining of different epithelia. Tumors Specifically Detected: Primary and metastatic carcinoma and meningioma. Polypeptides Reacting: Desmoplakin 1 and 2 (Mr 250 000 and 215 000). Reactivity on Tested Cell Lines: Several Human carcinoma cell lines: MCF-7, A-431, Rat MHC1C1 cell line, Bovine cells: MDBK, BMGE.

Réactivité croisée (Details): Species reactivity (tested):Human, Bovine, Rat, Mouse and Chicken.

Purification: Affinity Chromatography on Protein A

Immunogène: Bovine Desmoplakin 1 and 2

Isotype: IgG1

Information d'application

Indications d'application: Immunoblotting. Immunofluorescence. Immunohistochemistry on Frozen Sections: Dilute 1/10 with PBS, pH 7.4 (Incubation Time: 1 h at RT).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in 1 mL dist. water.

Buffer: Final solution contains PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody (in aliquots) at -20 °C.

Détails sur Desmoplakin

Antigène: Desmoplakin (DSP)

Autre désignation: Desmoplakin

Sujet: Desmosomes are intercellular junctions that form tight links between adjacent cells. Desmoplakin is an obligate component of functional desmosomes that attaches intermediate filaments to desmosomal plaques. It is involved in the organization of desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains. The N-terminus of desmoplakin is essential for localisation to the desmosome and interaction with plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds to intermediate filaments. The central region of desmoplakin comprises a coiled-coil rod domain that mediates homodimerisation. There are two isoforms of desmoplakin - desmoplakin I, which is an obligate component of all desmosomes, and desmoplakin II, which is predominantly expressed in tissues and cells of stratified origin. Mutations in the gene encoding desmoplakin result in a number of cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus.Synonyms: 250/210 kDa paraneoplastic pemphigus antigen, DP, DSP

ID gène: 1832

NCBI Accession: NP_001008844

UniProt: P15924

Pathways: Cell-Cell Junction Organization