Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
Specificité
This antibody reacts to Desmoplakin 1&2. It shows distinct punctate membrane staining of different epithelia. Tumors Specifically Detected: Primary and metastatic carcinoma and meningioma. Polypeptides Reacting: Desmoplakin 1 and 2 (Mr 250 000 and 215 000). Reactivity on Tested Cell Lines: Several Human carcinoma cell lines: MCF-7, A-431, Rat MHC1C1 cell line, Bovine cells: MDBK, BMGE.
Réactivité croisée (Details)
Species reactivity (tested):Human, Bovine, Rat, Mouse and Chicken.
DSP
Reactivité: Humain
ELISA
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Immunoblotting. Immunofluorescence. Immunohistochemistry on Frozen Sections: Dilute 1/10 with PBS, pH 7.4 (Incubation Time: 1 h at RT). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Desmosomes are intercellular junctions that form tight links between adjacent cells. Desmoplakin is an obligate component of functional desmosomes that attaches intermediate filaments to desmosomal plaques. It is involved in the organization of desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains. The N-terminus of desmoplakin is essential for localisation to the desmosome and interaction with plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds to intermediate filaments. The central region of desmoplakin comprises a coiled-coil rod domain that mediates homodimerisation. There are two isoforms of desmoplakin - desmoplakin I, which is an obligate component of all desmosomes, and desmoplakin II, which is predominantly expressed in tissues and cells of stratified origin. Mutations in the gene encoding desmoplakin result in a number of cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus.Synonyms: 250/210 kDa paraneoplastic pemphigus antigen, DP, DSP