Transferrin Receptor anticorps

N° du produit ABIN111936

Détail du produit anti-Transferrin Receptor anticorps

Antigène: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Transferrin Receptor est non-conjugé

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography.

Immunogène: KG-1 cells

Clone: VIP-1

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry on Frozen Sections: 2-4 μg/mL (1/50-1/100). Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water (= 0.2 mg/mL stock solution).

Concentration: 0.2 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 0.02 % Sodium Azide as preservative and 10 mg/mL BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: All human source materials have tested negative for HIV1, HIV2, HBc, HCV and HBsAg antibodies. No test guarantees a product to be non-infectious. Therefore, all material derived from human fluids or tissues should be considered as potentially infectious.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: RT/4 °C/-20 °C

Stockage commentaire: Lyophilized: Store at 2-8 °C (preferably in a dessicator). Reconstituted: Aliquot and store at -20 °C.

Détails sur Transferrin Receptor

Antigène: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Autre désignation: CD71 / TFRC (TFRC Produits)

Synonymes: anticorps Trfr, anticorps LOC100219280, anticorps tfr, anticorps cd71, anticorps tfr1, anticorps trfr, anticorps CD71, anticorps T9, anticorps TFR, anticorps TFR1, anticorps TR, anticorps TRFR, anticorps p90, anticorps 2610028K12Rik, anticorps AI195355, anticorps AI426448, anticorps AU015758, anticorps E430033M20Rik, anticorps Mtvr-1, anticorps Mtvr1, anticorps transferrin receptor, anticorps TFRC, anticorps Tfrc, anticorps tfrc

Sujet: CD71 is a homodimeric membrane glycoprotein consisiting of two disulfide-bonded 95kD chains. It is a type II membrane protein with a short aminoterminal cytoplasmic domain of 61 amino acid residues which mediates internalization and recycling, a single transmembrane region of 28 amino acid residues, and a large extracellular transferrin-binding domain of 671 residues. Other significant structural features include N-linked and O-linked glycans and the covalent attachment of palmitic acid to cystein residues close to the cytoplasmic face of the membrane.Synonyms: TfR1, Transferrin receptor protein 1, p90

ID gène: 9606

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis