Insulin anticorps

N° du produit ABIN111963

Cet anticorps anti-Insulin est un anticorps Souris Monoclonal détectant Insulin dans IHC (p), IHC (fro) et EIA. Adapté pour Humain, Porc et Boeuf (Vache).

Aperçu rapide pour Insulin anticorps (ABIN111963)

Antigène: Insulin (INS)

Reactivité: Humain, Porc, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Insulin est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: IN-05

Détail du produit anti-Insulin anticorps

Specificité: The antibody IN-05 is specific for Human insulin. It recognizes also porcine and bovine insulin. No cross-reactions were found with other serum proteins. The antibody can be used for the quantification of human insulin by RIA, and also for receptor-insulin interactions. In normal tissues, IN-05 reacts with normal pancreatic islet beta cells. In tumor tissues it stains insulin secreting neoplasms (insulinomas).

Purification: Affinity Chromatography.

Isotype: IgG1

Information d'application

Indications d'application: Suitable for Immunohistochemistry (IHC), RIA and Receptor Studies. Recommended Dilutions for IHC: Frozen sections: 0.2 μg/mL (1/1000)Paraffin sections: 0.2 μg/mL (1/1000). Pretreatment not necessary. Suggested positive control: Human pancreas.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 with 5 mg/mL BSA as stabilizer and 09 % Sodium Azide as a preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur Insulin

Antigène: Insulin (INS)

Autre désignation: Insulin

Sujet: Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.Synonyms: INS

ID gène: 3630

NCBI Accession: NP_000198

UniProt: P01308

Pathways: Signalisation NF-kappaB, Signalisation RTK, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation