Crossreactivity < 1 % to other collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Réactivité croisée (Details)
Species reactivity (tested):Human, Mouse, Rat, Bovine, Sheep.
Attributs du produit
Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule
Purification
Immunoaffinity Chromatography using immobilised antigens followed by extensive cross-adsorbtion against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Immunogène
Collagen type II purified from adult Human knee and Bovine nasal cartilage. For immunization collagens extensively purified by limited pepsin digestion and selective salt precipitation were used. This preparation results in a native conformation of the protein.
COL2A1
Reactivité: Humain
IHC, ELISA
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for immunoprecipitation and for Western blottingfor highly sensitive qualitative analysis. Recommended dilutions: ELISA 1: 10,000 - 1: 50,000Western blot 1: 5,000 - 1: 10,000Immunohistochemistry 1: 200 - 1: 1,000
Restrictions
For Research Use only
Concentration
1.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.1 M Sodium Borate, 0.071 M Sodium Chloride, 0.001 M EDTA, pH 8.0, 0.01 % Sodium Azide, 10 mg/mL BSA (IgG and Protease free)
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Dilute only prior to immediate use
Stock
4 °C/-20 °C
Stockage commentaire
This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
anticorps ANFH, anticorps AOM, anticorps COL11A3, anticorps SEDC, anticorps Col2, anticorps Col2a, anticorps Col2a-1, anticorps Del1, anticorps Dmm, anticorps Lpk, anticorps M100856, anticorps Rgsc856, anticorps CG2A1A, anticorps COLLII, anticorps col2a1a, anticorps CB11, anticorps col2a1, anticorps coll2a1, anticorps fb38c06, anticorps fc10c01, anticorps wu:fb38c06, anticorps wu:fc10c01, anticorps collagen type II alpha 1 chain, anticorps collagen, type II, alpha 1, anticorps collagen, type II, alpha 1 L homeolog, anticorps collagen, type II, alpha 1 S homeolog, anticorps collagen, type II, alpha 1a, anticorps COL2A1, anticorps Col2a1, anticorps col2a1, anticorps col2a1.L, anticorps col2a1.S, anticorps col2a1a
Sujet
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.Synonyms: Alpha-1 type II collagen, COL2A1