ISG15 anticorps

N° du produit ABIN116955

Détail du produit anti-ISG15 anticorps

Antigène: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ISG15 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Purification: A multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated below

Immunogène: Recombinant human ISG15 protein

Information d'application

Indications d'application: This purified polyclonal antibody reacts with human ISG15 by ELISA and Western blot. This antibody using the specified conditions may recognize other prominent intrinsic

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.1 mL of deionized water or equivalent.

Concentration: 5.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 2-8 °C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20 °C or below.

Détails sur ISG15

Antigène: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Autre désignation: ISG15 / G1P2 (ISG15 Produits)

Synonymes: anticorps G1P2, anticorps IFI15, anticorps IP17, anticorps UCRP, anticorps hUCRP, anticorps ISG15, anticorps g1p2, anticorps si:rp71-1c23.2, anticorps G1p2, anticorps IGI15, anticorps Irfp, anticorps ISG17, anticorps ISG17/UCRP, anticorps ISG15 ubiquitin-like modifier, anticorps ISG15, anticorps isg15, anticorps Isg15

Sujet: Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. ISG15 (Interferon Stimulating Gene-15) shows no amino acid sequence homology to cytokines and is synthesized as a precursor that is activated through processing by a thiol protease. ISG15 is secreted by monocytes and lymphocytes. Synthesis is induced in response to IFN-α or IFN-β or IFN-omega, but not IFN-γ. ISG15 expression is induced also by overexpression of some interferon regulatory factors that have been shown to play a role in the transcriptional regulation of IFN genes. ISG15 is secreted also by cell lines of monocyte (U937 cell line), T-lymphocyte, B-lymphocyte (DAUDI cells), human fibroblasts, and epithelial origins. The induction of terminal differentiation in human melanoma cells is associated, among other things, with alterations in the expression of ISG15. Intracellularly ISG15 has been shown to function as a ubiquitin homologue. It is known also as UCRP (ubiquitin cross-reactive protein). Serpin 2a (spi2a), a member of the serine protease inhibitor (serpin) protein family that is highly induced in macrophages during bacillus Calmette-Guerin infection has been shown to bind ISG15. ISG15 has been shown to modulate immune cell function. It possesses activities of cytokines and induces production of IFN-γ. It enhances proliferation and functions of natural killer and LAK cells.Synonyms: Interferon-induced 15 kDa protein, Interferon-induced 17 kDa protein, UCRP, Ubiquitin cross-reactive protein

ID gène: 9636

NCBI Accession: NP_005092

UniProt: P05161