SYK
Reactivité: Humain
WB, ELISA, IHC, IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
This antibody conjugate is suitable for intracellular staining of human lymphoid cell lines, peripheral blood mononuclear cells, and mouse splenocytes using BD Phosflow™ Fix Buffer I or Lyse/Fix Buffer. Any of the three BD™ Phosflow permeabilization buffers may be used.
Volume d'échantillon
20 μL
Restrictions
For Research Use only
Format
Liquid
Buffer
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
Abtahian, Guerriero, Sebzda, Lu, Zhou, Mocsai, Myers, Huang, Jackson, Ferrari, Tybulewicz, Lowell, Lepore, Koretzky, Kahn: "Regulation of blood and lymphatic vascular separation by signaling proteins SLP-76 and Syk." dans: Science (New York, N.Y.), Vol. 299, Issue 5604, pp. 247-51, (2003) (PubMed).
Hong, Yankee, Harrison, Geahlen: "Regulation of signaling in B cells through the phosphorylation of Syk on linker region tyrosines. A mechanism for negative signaling by the Lyn tyrosine kinase." dans: The Journal of biological chemistry, Vol. 277, Issue 35, pp. 31703-14, (2002) (PubMed).
Zhang, Berenstein, Siraganian: "Phosphorylation of Tyr342 in the linker region of Syk is critical for Fc epsilon RI signaling in mast cells." dans: Molecular and cellular biology, Vol. 22, Issue 23, pp. 8144-54, (2002) (PubMed).
Latour, Veillette: "Proximal protein tyrosine kinases in immunoreceptor signaling." dans: Current opinion in immunology, Vol. 13, Issue 3, pp. 299-306, (2001) (PubMed).
Vines, Potter, Xu, Geahlen, Costello, Tybulewicz, Lowell, Chang, Gresham, Willman: "Inhibition of beta 2 integrin receptor and Syk kinase signaling in monocytes by the Src family kinase Fgr." dans: Immunity, Vol. 15, Issue 4, pp. 507-19, (2001) (PubMed).
Syk is a non-receptor protein-tyrosine kinase that is closely related to ZAP70 and plays crucial roles in the development and receptor-mediated signaling of most leukocytes and in vascular integrity. Syk is expressed in hematopoietic cells, including B lymphocytes, immature (CD4, CD8 double-negative and double-positive) thymocytes, and myeloid cells, epithelial cell lines, and normal breast tissue. Mature (CD4 or CD8 single-positive) thymocytes and peripheral alphabeta TCR-bearing T lymphocytes have very low or undetectable levels of Syk. Syk contributes to the signal transduction process by binding to ITAMs (Immunoreceptor Tyrosine-based Activation Motifs) of immune receptors, including Igalpha and Igbeta (CD79a and b), TCRzeta, CD3epsilon, and FcRgamma. Upon receptor activation, Syk binds to phosphorylated ITAMs via its two N-terminal SH2 domains thereby activating Syk and causing tyrosines in the interdomain, between the SH2 and Kinase domains of Syk, to undergo auto-phosphorylation and phosphorylation by Lyn. The tyrosine 348 phosphorylation site (pY348) in human Syk is orthologous to tyrosine 342 in mouse and rat Syk and tyrosine 315 in human ZAP70. This phosphorylated site can act as a binding site for other signaling molecules, such as PLCgamma, Vav, and Fgr. The I120-772 antibody is specific for human Syk (pY348) and does not cross-react with phosphorylated Zap70. The orthologous phosphorylation site in mouse and rat Syk is Y342.