SUMO3 anticorps

N° du produit ABIN118003

Détail du produit anti-SUMO3 anticorps

Antigène: SUMO3 (Small Ubiquitin Related Modifier 3 (SUMO3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SUMO3 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Réactivité croisée (Details): Species reactivity (tested):Human

Purification: Multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis

Immunogène: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant human SUMO-3 protein.

Information d'application

Indications d'application: ELISA. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.1 mL of deionized water (or equivalent).

Concentration: 5.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 2-8 °C prior to restoration. For extended storage mix with glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.

Détails sur SUMO3

Antigène: SUMO3 (Small Ubiquitin Related Modifier 3 (SUMO3))

Autre désignation: SUMO3 / SMT3A (SUMO3 Produits)

Synonymes: anticorps SMT3A, anticorps SMT3H1, anticorps SUMO-3, anticorps Smt3B, anticorps 2810014B19Rik, anticorps AA409334, anticorps AW121497, anticorps AW536077, anticorps D10Ertd345e, anticorps Smt3h1, anticorps smt3a, anticorps smt3h1, anticorps fa94a11, anticorps im:7141764, anticorps sumo2, anticorps sumo3, anticorps sumo3l, anticorps wu:fa94a11, anticorps wu:fb55d09, anticorps zgc:65847, anticorps zgc:85643, anticorps fc66f10, anticorps fl11h12, anticorps wu:fc66f10, anticorps wu:fl11h12, anticorps zgc:86902, anticorps SUMO3, anticorps ATSUMO3, anticorps MCO15.12, anticorps MCO15_12, anticorps SMALL UBIQUITIN-LIKE MODIFIER 3, anticorps SUM3, anticorps SUMO 3, anticorps small ubiquitin-like modifier 3, anticorps GMP1, anticorps PIC1, anticorps SENTRIN, anticorps SMT3, anticorps SMT3H3, anticorps SMTP3, anticorps SUMO-1, anticorps Smt3C, anticorps Ubl1, anticorps small ubiquitin-like modifier 3, anticorps small ubiquitin-like modifier 3 L homeolog, anticorps small ubiquitin-like modifier 3a, anticorps small ubiquitin-like modifier 3b, anticorps small ubiquitin-related modifier 2 pseudogene, anticorps SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae), anticorps small ubiquitin-like modifier 2, anticorps small ubiquitin-like modifier 1, anticorps SUMO3, anticorps Sumo3, anticorps sumo3.L, anticorps sumo3a, anticorps sumo3b, anticorps LOC490297, anticorps sumo3, anticorps sumo2, anticorps Sumo1

Sujet: Covalent modification of cellular proteins by the ubiquitin-like modifier SUMO (small ubiquitin-like modifier) regulates various cellular processes, such as nuclear transport, signal transduction, stress responses and cell cycle progression. But, in contrast to ubiquination, sumoylation does not tag proteins for degradation by the 26S proteasome, but rather seems to enhance stability or modulate their subcellular compartmentalization. Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA interactions, as well as localization and stability of the target protein. Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to human. Where invertebrates have only a single SUMO gene termed SMT3, three members of the SUMO family have been identified in vertebrates: SUMO-1 and the close homologues SUMO-2 and SUMO-3. SUMO has been called SMT3 (yeast), sentrin, PIC1, GMP1 and UBL1. SUMO has been shown to bind and regulate mammalian SP-RINGs (such as Mdm2, PIAS and PML), RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c- Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, WRN, Sp100, IκB-α, Androgen receptor (AR), GLUT1/4, Drosophila Ttk69, Dorsal, CaMK, yeast Septins, and viral CMV-IE1/2, EBV-BZLF1, HPV/BPV-E1. These bindings implicate SUMO in the stabilization of the target proteins and/or their localization to subcellular complexes. SUMO has an apparent molecular weight of ~12 kDa and human SUMO-1 (a 101 amino acid polypeptide) shares 50 % sequence identity with SUMO-2 and SUMO-3 and with yeast SMT3. SUMO and ubiquitin only show about 18 % homology, but both possess a common three-dimensional structure characterized by a tightly packed globular fold with β-sheets wrapped around an α-helix.Synonyms: SMT3 homolog 1, SMT3H1, SUMO-3, Small ubiquitin-related modifier 3, Ubiquitin-like protein SMT3A

ID gène: 6612

NCBI Accession: NP_008867

UniProt: P55854