c-MYC anticorps (C-Term) (FITC)

N° du produit ABIN119682

Cet anticorps anti-c-MYC Monoclonal Souris conjugué à FITC (Clone 9-00E-010) (ABIN119682) détecte spécifiquement c-MYC dans IF, FACS et IHC (fro). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour c-MYC anticorps (C-Term) (FITC) (ABIN119682)

Antigène: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp c-MYC est conjugé à/à la FITC

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 9-00E-010

Détail du produit anti-c-MYC anticorps (FITC)

Épitope: C-Term

Séquence: EQKLISEEDL

Specificité: This antibody detects the 62 kDa c-myc gene product, which is involved in the regulation of the cell cycle and cell growth. This antibody may also be used to detect the commonly used c-myc tag.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G.

Immunogène: Synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-terminal region of Human c-myc. Spleen cells from immunised Balb/c mice were fused with cells of the myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells in 100 μL. Membranepermeabilisation is required for this application. Immunoflourescence. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur c-MYC

Antigène: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Autre désignation: C-Myc

Sujet: P62c-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers.Synonyms: c-myc tag, myc tag, myc-tag

ID gène: 4609

NCBI Accession: NP_002458

UniProt: P01106

Pathways: Signalisation p53, Cycle Cellulaire, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, L'effet Warburg