p53 anticorps (AA 20-25) (Biotin)

N° du produit ABIN120052

Cet anticorps Souris Monoclonal détecte spécifiquement p53 dans FACS. Il présente une réactivité avec des échantillons de Humain, Boeuf (Vache), Cheval et Cat.

Aperçu rapide pour p53 anticorps (AA 20-25) (Biotin) (ABIN120052)

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivité: Humain, Boeuf (Vache), Cheval, Cat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp p53 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Clone: DO-1

Détail du produit anti-p53 anticorps (Biotin)

Épitope: AA 20-25

Specificité: This antibody recognizes the p53 tumour suppressor protein, binding to both wild type and mutant forms. Clone DO-1 binds to both wild type and mutant forms of the p53 protein found in various malignancies (Kern et al. 1992). p53 is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells (Haupt et al. 2003) and thus functions as a tumor suppressor that is involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancer (Joerger and Fersht 2007). DO-1 recognizes an epitope at the N-terminal end of p53 between amino acids 20-25, common to isoforms 1-3 of p53.

Réactivité croisée (Details): Species reactivity (tested):Human, Horse, Bovine, Cat, Green Monkey and Sheep.

Purification: Affinity chromatography on Protein G

Immunogène: Recombinant human p53. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.

Isotype: IgG2a

Information d'application

Indications d'application: Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 1 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur p53

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: p53 (TP53)

Sujet: Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

ID gène: 7157

NCBI Accession: NP_000537

UniProt: P04637

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg