PAG1 anticorps (C-Term)

N° du produit ABIN120704

L’anticorps anti-PAG1 Monoclonal Souris est utilisé pour la détection de PAG1 dans des échantillons de Humain, Souris, Rat et Boeuf (Vache). Il a été validé pour IP et IHC (p).

Aperçu rapide pour PAG1 anticorps (C-Term) (ABIN120704)

Antigène: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PAG1 est non-conjugé

Application: Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PAG-C1

Détail du produit anti-PAG1 anticorps

Épitope: C-Term

Specificité: The antibody recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.

Purification: Protein-A affinity chromatography

Pureté: > 95 % pure by SDS-PAGE

Immunogène: C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH

Isotype: IgG2b

Information d'application

Indications d'application: Immunoprecipitation. Positive control: RAJI human Burkitt lymphoma cell line.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Détails sur PAG1

Antigène: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Autre désignation: PAG1 / Csk-Binding Protein

Sujet: PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.Synonyms: CBP, PAG, Phosphoprotein associated with glycosphingolipid-enriched microdomains 1, Transmembrane adapter protein PAG, Transmembrane phosphoprotein Cbp

ID gène: 55824

UniProt: Q9NWQ8

Pathways: Signalisation p53, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events