PRKACA anticorps (Catalytic Subunit)

N° du produit ABIN127012

Détail du produit anti-PRKACA anticorps

Antigène: PRKACA (Protein Kinase A, alpha (PRKACA))

Épitope: Catalytic Subunit

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Mouton, Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRKACA est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificité: Recognizes a 40 kDa PKA on SDS-PAGE immunoblots

Purification: Affinity purified

Immunogène: Synthetic peptide surrounding amino acid 11 of the catalytic subunit of PKA alpha isoform.

Information d'application

Indications d'application: Western blot: 0.5-4 μg/mL, Jukat cell lysate can be used as a positive control. Immunoprecipitation: 20-30 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C/-80 °C

Stockage commentaire: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Détails sur PRKACA

Antigène: PRKACA (Protein Kinase A, alpha (PRKACA))

Autre désignation: PRKACA (PRKACA Produits)

Synonymes: anticorps PKACA, anticorps Calpha, anticorps Cs-PKA, anticorps PKCA1, anticorps PKA, anticorps PKCD, anticorps Pkaca, anticorps zgc:158799, anticorps protein kinase cAMP-activated catalytic subunit alpha, anticorps protein kinase, cAMP dependent, catalytic, alpha, anticorps cAMP-dependent protein kinase catalytic subunit, anticorps protein kinase, cAMP-dependent, catalytic, alpha, genome duplicate a, anticorps PRKACA, anticorps Prkaca, anticorps kin-1, anticorps prkacaa

Sujet: Protein kinase A (PKA) also known as cAMP-dependent protein kinase, is a 40 kDa protein kinase that phosphorylates serine or threonine residues in target proteins in response to elevated levels of cAMP. The second messenger cAMP is produced in response to a wide variety of hormones & neurotransmitters and regulates cellular processes such as cell growth and differentiation, ion channel conductivity, synaptic release of neurotransmitters & gene transcription. The principle intracellular target for cAMP is PKA. Inactive PKA exists as a tetrameric protein composed of two regulatory (R) subunits and two catalytic (C) subunits. Activation occurs when two cAMP molecules bind to each R subunit which causes a conformational change that releases the active C subunits. Two major PKA isozymes, type I (PKAI) and type II (PKAII) consist of RI and RII respectively, complexed with C. Three different C subunits (alpha, beta, and gamma) and four different R subunits (RI-alpha, RI-beta, RII-alpha, and RII-beta) have been identified. It has been suggested that the complex structure of PKA may be necessary for it to accomplish such diverse functions.Synonyms: PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha

ID gène: 5566

NCBI Accession: NP_002721

UniProt: P17612

Pathways: Signalisation NF-kappaB, Signalisation Hedgehog, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, Lipid Metabolism, SARS-CoV-2 Protein Interactome, Phosphorylation & l'infection par le SRAS-CoV-2