MEK1/2 anticorps

N° du produit ABIN1302344

Détail du produit anti-MEK1/2 anticorps

Antigène: MEK1/2 (MAP2K1/2) (Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MEK1/2 est non-conjugé

Application: Western Blotting (WB)

Specificité: The polyclonal antibody reacts with intracellular antigens MEK 1/2. MEK 1 and MEK 2 are integral components of the MAP kinase cascade, an important pathway for cell growth and differentiation.

Purification: Purified by ligand affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Synthetic peptide (coupled with KLH) derived from aminoacid sequence 33-53 of human MAPK Kinase 1. The immunogen sequence is highly conserved within species (human, mouse, rat, hamster, bovine etc.).

Isotype: IgG

Information d'application

Indications d'application: Western blotting: Recommended dilution 0.5 μg/mL, positive material: RAJI human Burkitt lymphoma cell line, both reducing and non-reducing conditions, reducing conditions are recommended.

Commentaires:

Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Human), Phosphotyrosine)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Détails sur MEK1/2

Antigène: MEK1/2 (MAP2K1/2) (Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2))

Autre désignation: MAP2K1/2 (MAP2K1/2 Produits)

Sujet: MEK (MAPKK, kinase of mitogen-activated protein kinase) is the medium one of three components of MAP kinase cascade –, an important signaling pathway that regulates cell growth and differentiation. Raf (MAPKKK) activates MEK 1 and 2 via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK 1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK (MAPK, mitogen-activated protein kinase). This phosphorylation of ERK by MEK 1/2 is a critical step in the MAP kinase cascade. Phosphorylated ERK is capable of translocating to the nucleus, where it regulates gene expression by activating transcription factors.,MAPKK 1/2, MKK1/2