PKM2 anticorps (AA 405-409)

N° du produit ABIN1355885

Détail du produit anti-PKM2 anticorps

Antigène: PKM2 (Pyruvate Kinase M2 (PKM2))

Épitope: AA 405-409

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PKM2 est non-conjugé

Application: Western Blotting (WB)

Specificité: The antibody detects endogenous levels of total PKM2 protein.

Immunogène: Peptide sequence around AA 405-409 (T-S-D-P-T) derived from Human PKM2. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Information d'application

Indications d'application: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-PKM2 (ABIN1355885)

Jiang, Li, Yang, Hawke, Zheng, Xia, Aldape, Wei, Guo, Chen, Lu: "PKM2 regulates chromosome segregation and mitosis progression of tumor cells." dans: Molecular cell, Vol. 53, Issue 1, pp. 75-87, (2014) (PubMed).

Yang, Xia, Hawke, Li, Liang, Xing, Aldape, Hunter, Alfred Yung, Lu: "PKM2 phosphorylates histone H3 and promotes gene transcription and tumorigenesis." dans: Cell, Vol. 150, Issue 4, pp. 685-96, (2012) (PubMed).

Yang, Zheng, Xia, Ji, Chen, Guo, Lyssiotis, Aldape, Cantley, Lu: "ERK1/2-dependent phosphorylation and nuclear translocation of PKM2 promotes the Warburg effect." dans: Nature cell biology, Vol. 14, Issue 12, pp. 1295-304, (2012) (PubMed).

Détails sur PKM2

Antigène: PKM2 (Pyruvate Kinase M2 (PKM2))

Autre désignation: PKM2 (PKM2 Produits)

Synonymes: anticorps CTHBP, anticorps OIP3, anticorps PK3, anticorps PKM2, anticorps TCB, anticorps THBP1, anticorps AA414905, anticorps AL024370, anticorps AL024424, anticorps Pk-2, anticorps Pk-3, anticorps Pk3, anticorps Pkm2, anticorps PKM12, anticorps PKM, anticorps pyruvate kinase M1/2, anticorps pyruvate kinase, muscle, anticorps PKM, anticorps Pkm

Sujet: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Poids moléculaire: 60 kDa

NCBI Accession: NP_872270

Pathways: L'effet Warburg