p53 anticorps (AA 1-393)

N° du produit ABIN1386190

Cet anticorps Lapin Polyclonal détecte spécifiquement p53 dans WB, ELISA, FACS, IHC (p), ICC, IF (p), IF (cc) et IHC (fro). Il présente une réactivité envers Humain, Souris et Rat et a été mentionné dans 4+ publications.

Aperçu rapide pour p53 anticorps (AA 1-393) (ABIN1386190)

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp p53 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-p53 anticorps

Épitope: AA 1-393

Réactivité croisée: Humain, Souris, Rat

Homologie: Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogène: Full length human p53 protein

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-p53 (ABIN1386190)

Sola, Magrin, Pedrioli, Pinton, Salvadè, Papin, Paganetti: "Tau affects P53 function and cell fate during the DNA damage response." dans: Communications biology, Vol. 3, Issue 1, pp. 245, (2021) (PubMed).

Zhang, Zhao, Zhang, Hao, Yu, Min, Li, Ma, Chen, Yi, Tang, Meng, Liu, Wang, Shen, Zhang: "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." dans: Chemosphere, Vol. 194, pp. 147-157, (2018) (PubMed).

Zeng, Feng, Lin, Ma, Liu: "Effects of microRNA-211 on proliferation and apoptosis of lens epithelial cells by targetingSIRT1gene in diabetic cataract mice." dans: Bioscience reports, Vol. 37, Issue 4, (2017) (PubMed).

Qin, Xiong, Liu, Yao, Zhou, Hua, Wang: "Effects of irreversible electroporation on cervical cancer cell lines in vitro." dans: Molecular medicine reports, Vol. 14, Issue 3, pp. 2187-93, (2016) (PubMed).

Détails sur p53

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: p53

Sujet:

Synonyms: LFS1, p53, p53 Cellular Tumor Antigen, p53 Tumor Suppressor, Phosphoprotein p53, TP53, Transformation related protein 53, TRP53, Tumor protein p53, Tumour Protein p53.

Background: p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.

ID gène: 7157

UniProt: P04637

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg