ATP6V1A anticorps

N° du produit ABIN1573979

Détail du produit anti-ATP6V1A anticorps

Antigène: ATP6V1A (ATPase, H+ Transporting, Lysosomal 70kDa, V1 Subunit A (ATP6V1A))

Reactivité: Humain, Souris, Boeuf (Vache), Poulet, Drosophila melanogaster, Poisson, Manduca, Nicotiana tabacum, Orang-Utan

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATP6V1A est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: Rabbit Anti-V-ATPase Subunit A Polyclonal Antibody reacts with mouse V-ATPase subunit A. Sequence homology predicts that it will also react with human, bovine, orangutan, tobacco hornworm, chicken, fruit fly, and zebrafish V-ATPase subunit A.

Réactivité croisée (Details): Rabbit Anti-V-ATPase Subunit A Polyclonal Antibody reacts with mouse V-ATPase subunit A. Sequence homology predicts that it will also react with human, bovine, orangutan, tobacco hornworm, chicken, fruit fly, and zebrafish V-ATPase subunit A.

Purification: Immunoaffinity chromatography

Immunogène: Synthetic peptide conjugated - KLH

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.02-0.2 µg/mL
Western blot: 1.0 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS, pH 7.4, containing 30 % glycerol, and 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freezing and thawing cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable for 2-3 weeks if stored at 2-8 °C. For long term storage, aliquot and store at -28 °C or below.

Références pour anticorps anti-ATP6V1A (ABIN1573979)

Gokey, Dasgupta, Amack: "The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish." dans: Developmental biology, Vol. 407, Issue 1, pp. 115-30, (2016) (PubMed).

Grubman, Lidgerwood, Duncan, Bica, Tan, Parker, Caragounis, Meyerowitz, Volitakis, Moujalled, Liddell, Hickey, Horne, Longmuir, Koistinaho, Donnelly, Crouch, Tammen, White, Kanninen: "Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder." dans: Acta neuropathologica communications, Vol. 2, pp. 25, (2014) (PubMed).

Monteiro, Aires, Becker, Jacinto, Certal, Rodríguez-León: "V-ATPase proton pumping activity is required for adult zebrafish appendage regeneration." dans: PLoS ONE, Vol. 9, Issue 3, pp. e92594, (2014) (PubMed).

Li, Chen, Klemmer, Koopmans, Karupothula, Smit: "Identifying true protein complex constituents in interaction proteomics: the example of the DMXL2 protein complex." dans: Proteomics, Vol. 12, Issue 15-16, pp. 2428-32, (2012) (PubMed).

Nylandsted, Becker, Bunkenborg, Andersen, Dengjel, Jäättelä: "ErbB2-associated changes in the lysosomal proteome." dans: Proteomics, Vol. 11, Issue 14, pp. 2830-8, (2011) (PubMed).

Détails sur ATP6V1A

Antigène: ATP6V1A (ATPase, H+ Transporting, Lysosomal 70kDa, V1 Subunit A (ATP6V1A))

Autre désignation: V-ATPase Subunit A (ATP6V1A Produits)

Synonymes: anticorps ATP6A1, anticorps ATP6V1A1, anticorps HO68, anticorps VA68, anticorps VPP2, anticorps Vma1, anticorps AI647066, anticorps Atp6a1, anticorps Atp6a2, anticorps Atp6v1a1, anticorps ATP6V1A, anticorps atp6v1al, anticorps zgc:63516, anticorps An02g10440, anticorps AO090102000349, anticorps vacuolar ATP synthase subunit A, anticorps atp6a1, anticorps atp6v1a1, anticorps ho68, anticorps v1a, anticorps va68, anticorps vma1, anticorps vpp2, anticorps CG12403, anticorps Dmel\CG12403, anticorps V-ATPase, anticorps Vha, anticorps Vha-68-1, anticorps Vha68, anticorps vha67-2, anticorps vha68-1, anticorps ATPase H+ transporting V1 subunit A, anticorps ATPase, H+ transporting, lysosomal V1 subunit A, anticorps ATPase, H+ transporting, lysosomal, V1 subunit Aa, anticorps v-type proton ATPase catalytic subunit A, anticorps vacuolar ATP synthase subunit A, anticorps ATPase H+ transporting V1 subunit A L homeolog, anticorps vacuolar proton pump 3, anticorps Vacuolar H[+] ATPase 68kD subunit 1, anticorps ATP6V1A, anticorps Atp6v1a, anticorps atp6v1aa, anticorps ANI_1_1468024, anticorps AOR_1_602134, anticorps VHA-A, anticorps atp6v1a.L, anticorps vpp3, anticorps Vha68-1

Sujet: The V-ATPase subunit A is a catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.Rabbit Anti-V-ATPase Subunit A Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide, purified from rabbit antiserum by immunoaffinity chromatography, and supplied as 40 ug aliquot at concentration of 0.5 mg/ml.

Pathways: Transition Metal Ion Homeostasis, Proton Transport, SARS-CoV-2 Protein Interactome