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Pepsin anticorps (Biotin)

Reactivité: Porc WB, IHC, ELISA Hôte: Chèvre Polyclonal Biotin
N° du produit ABIN1607675
  • Antigène Voir toutes Pepsin Anticorps
    Pepsin
    Reactivité
    • 15
    • 14
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Porc
    Hôte
    • 19
    • 6
    • 2
    • 1
    Chèvre
    Clonalité
    • 26
    • 2
    Polyclonal
    Conjugué
    • 16
    • 9
    • 2
    • 1
    Cet anticorp Pepsin est conjugé à/à la Biotin
    Application
    • 27
    • 12
    • 11
    • 5
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    Réactivité croisée (Details)
    Cross reactivity against Pepsin from other sources may occur but have not been specifically determined.
    Pureté
    Anti-Pepsin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach].
    niveau d'endotoxine
    Low Endotoxin : No
    Immunogène
    Pepsin [Porcine Stomach]
    Immunogen Type: Native Protein
    Isotype
    IgG
    Top Product
    Discover our top product Pepsin Anticorps primaire
  • Indications d'application
    Anti-Pepsin antibody is suitable for ELISA, Immunohisochemistry, and Western Blot. Expect band at 41.2 kDa in appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
    ELISA Dilution: 1:3.000 - 1:15.000
    Western Blot Dilution: 1:500 - 1:2.000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    10 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Conseil sur la manipulation
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Date de péremption
    Expiration date is one (1) year from date of opening.
  • Antigène
    Pepsin
    Abstract
    Pepsin Produits
    Sujet
    Pepsin antibody detects Pepsin protein. Pepsin is an enzyme whose pepsinogen is released by the chief cells in the stomach and degrades food proteins into peptides. Pepsin belongs to the peptidase A1 family and is one of three principal proteolytic enzymes in the digestive system. Pepsin is most efficient in cleaving peptide bonds between hydrophobic and preferably aromatic amino acids such as phenylalanine, tryptophan, and tyrosine at positions P1 and P1'. Anti-Pepsin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research. Synonyms: ec 3.4.23.1 antibody, Pepsin A antibody, Pepsin a precursor antibody, Pepsin antibody, Pepsinogen 1 second locus antibody, Pepsinogen 3 group 1 antibody, Pepsinogen a3 antibody
    ID gène
    396892
    UniProt
    P00791
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