BSA anticorps (Biotin)

N° du produit ABIN1607855

Détail du produit anti-BSA anticorps (Biotin)

Antigène: BSA (Bovine Serum Albumin (BSA))

Reactivité: Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BSA est conjugé à/à la Biotin

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Réactivité croisée (Details): Cross reactivity against Albumin from other sources may occur but have not been specifically determined.

Purification: Bovine serum antibody was purified as an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Albumin (Bovine Serum].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Albumin (Bovine Serum)
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-BSA antibody is suitable for detection of bovine serum albumin in biological samples by ELISA, western blot and immunohistochemisty assays. Specific dilutions are recommended but should be opitmized by researcher.
ELISA Dilution: 1:50.000
Immunohistochemistry Dilution: 1:300 - 1:2.500
Western Blot Dilution: 1:1.000 - 1:5.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur BSA

Antigène: BSA (Bovine Serum Albumin (BSA))

Abstract: BSA Produits

Synonymes: anticorps PRO0883, anticorps PRO0903, anticorps PRO1341, anticorps albumin, anticorps ALB

Sujet: Anti-BSA antibody is specific for the full-length BSA precursor protein is 607 amino acids in length. An N-terminal 18-residue signal peptide is cut off from the precursor protein upon secretion, and an additional 4 amino acids is cleaved to yield the mature BSA protein that contains 585 amino acids. BSA has numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry, and it is also used as a nutrient in cell and microbial culture. BSA is used as a stabilizer for some proteins and enzymes. This protein does not affect other enzymes that do not need it for stabilization. Synonyms: ALB antibody, Albumin antibody, Allergen Bos d 6 antibody, BSA antibody, DKFZp779N1935 antibody

ID gène: 280717

UniProt: P02769

Pathways: Lipid Metabolism