Alpha, beta Synuclein (AA 2-25), (N-Term) anticorps
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- Antigène
- Alpha, beta Synuclein
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Épitope
- AA 2-25, N-Term
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Reactivité
- Souris, Rat, Poisson zèbre (Danio rerio)
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Inconjugué
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- Specificité
- Recognizes alpha synuclein and beta synuclein.
- Purification
- antiserum
- Immunogène
- Synthetic peptide DVFMKGLSKAKEGVVAAAEKTKQG (aa 2-25 in alpha synuclein), coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
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- Indications d'application
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WB: 1 : 1000 (AP staining)
ICC: 1 : 500
IHC: 1 : 500 - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- PBS
- Conseil sur la manipulation
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Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable. - Stock
- 4 °C/-20 °C
- Stockage commentaire
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." dans: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).
: "Over-expression of alpha-synuclein in the nervous system enhances axonal degeneration after peripheral nerve lesion in a transgenic mouse strain." dans: Journal of neurochemistry, Vol. 114, Issue 4, pp. 1007-18, (2010) (PubMed).
: "Brain alpha-synuclein accumulation in multiple system atrophy, Parkinson's disease and progressive supranuclear palsy: a comparative investigation." dans: Brain : a journal of neurology, Vol. 133, Issue Pt 1, pp. 172-88, (2010) (PubMed).
: "Mitochondrial translocation of alpha-synuclein is promoted by intracellular acidification." dans: Experimental cell research, Vol. 314, Issue 10, pp. 2076-89, (2008) (PubMed).
: "alpha-Synuclein protects SH-SY5Y cells from dopamine toxicity." dans: Biochemical and biophysical research communications, Vol. 349, Issue 4, pp. 1294-300, (2006) (PubMed).
: "Molecular anatomy of a trafficking organelle." dans: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).
: "Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein." dans: Molecular and cellular biology, Vol. 25, Issue 4, pp. 1339-46, (2005) (PubMed).
: "
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Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." dans: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).
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- Antigène
- Alpha, beta Synuclein
- Autre désignation
- alpha/beta Synuclein
- Sujet
- Synonyms: alpha-Synuclein, beta-Synuclein
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