GABRA3 anticorps (AA 29-43)
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- Antigène Voir toutes GABRA3 Anticorps
- GABRA3 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3))
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Épitope
- AA 29-43
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Reactivité
- Rat, Souris
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp GABRA3 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- Specificité
- Specific for GABA-A receptor alpha3.
- Purification
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- Immunogène
- Synthetic peptide (aa 29-43 of rat GABA-A receptor alpha3 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
- Top Product
- Discover our top product GABRA3 Anticorps primaire
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- Indications d'application
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WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 500 - Commentaires
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WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Conseil sur la manipulation
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- Stock
- -20 °C
- Stockage commentaire
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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Long-term behavioral programming induced by peripuberty stress in rats is accompanied by GABAergic-related alterations in the Amygdala." dans: PLoS ONE, Vol. 9, Issue 4, pp. e94666, (2014) (PubMed).
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Long-term behavioral programming induced by peripuberty stress in rats is accompanied by GABAergic-related alterations in the Amygdala." dans: PLoS ONE, Vol. 9, Issue 4, pp. e94666, (2014) (PubMed).
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- Antigène
- GABRA3 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3))
- Autre désignation
- GABA-A Receptor Alpha3 (GABRA3 Produits)
- Synonymes
- anticorps GABRA3, anticorps Gabra-3, anticorps si:ch211-113k9.1, anticorps gamma-aminobutyric acid type A receptor alpha3 subunit, anticorps gamma-aminobutyric acid (GABA) A receptor, alpha 3, anticorps gamma-aminobutyric acid (GABA) A receptor, subunit alpha 3, anticorps gamma-aminobutyric acid receptor subunit alpha-3, anticorps GABRA3, anticorps gabra3, anticorps Gabra3, anticorps LOC100653496
- Sujet
- Synonyms: GABA(A) R alpha3
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