CD25 anticorps

N° du produit ABIN181143

Détail du produit anti-CD25 anticorps

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD25 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Enzyme Immunoassay (EIA)

Specificité: This antibody detects Human CD25.

Attributs du produit: Synonyms: Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TACantigen

Purification: Affinity Chromatography on Protein G

Immunogène: Highly pure (>98%) recombinant Human soluble IL-2 Receptor alpha

Isotype: IgG2a

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman sIL-2RA (10.0 μg/mL), a concentration of 1.0-1.5 μg/mL of this antibody is required,in the presence of 0.5 ng/mL recombinant hIL-2. ELISA: In a Sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of thisantibody will detect at least 100 pg/mL of recombinant Human sIL-2RA when used inconjunction with compatible secondary reagents. Western Blot: To detect Human sIL-2RA by Western Blot analysis this antibody can be usedat a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human sIL-2RA is 2.0-4.0 ng/lane, underreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2 - 8 °C for six weeks. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur CD25

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Autre désignation: CD25 / IL2RA (IL2RA Produits)

Synonymes: anticorps CD25, anticorps IDDM10, anticorps IL2R, anticorps TCGFR, anticorps IL2RAC, anticorps IL-2RA, anticorps Il2r, anticorps Ly-43, anticorps interleukin 2 receptor subunit alpha, anticorps interleukin-2 receptor subunit alpha-like, anticorps interleukin 2 receptor, alpha chain, anticorps IL2RA, anticorps Il2ra, anticorps LOC100222957

Sujet: The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55

ID gène: 3559

UniProt: P01589

Pathways: Signalistation JAK/STAT, Growth Factor Binding, Activated T Cell Proliferation