WNT3A
Reactivité: Humain
WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: Indirect: To detect hWNT-3a by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a. Sandwich: To detect hWNT-3a by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with Biotinylated Anti-HumanWNT-3a as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hWNT-3a. Western blot: 0.1 - 0.2 μg/mL (reducing and non-reducing conditions). Neutralisation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
Date de péremption
6 months
Antigène
WNT3A
(Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))