PRKAA1/PRKAA2 anticorps (pThr172, pThr174)

N° du produit ABIN1842476

Détail du produit anti-PRKAA1/PRKAA2 anticorps

Antigène: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Épitope: pThr172, pThr174

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRKAA1/PRKAA2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificité: AMPKalpha,1/AMPKalpha,2 Antibody detects endogenous levels of total AMPKalpha,1/AMPKalpha,2 protein.

Attributs du produit: Molecular Weight: Predicted Band Size 63 kDa, Observed Band Size 63 kDa

Purification: Rabbit Anti-AMPKalpha,1/AMPKalpha,2 Antibody (Ab-174/172) Polyclonal Antibody was purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized non-phosphopeptide derived from human AMPKalpha,1/AMPKalpha,2 around pThr174,pThr172 (L-R-TP-S-C).

Isotype: IgG

Information d'application

Indications d'application: IHC: 1:50-1:100,
WB: 1:500-1:1,000,
IF: 1:100-1:200

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide, and 50 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20 °C for 1 year.

Références pour anticorps anti-PRKAA1/PRKAA2 (ABIN1842476)

Arduini, Serviddio, Escobar, Tormos, Bellanti, Viña, Monsalve, Sastre: "Mitochondrial biogenesis fails in secondary biliary cirrhosis in rats leading to mitochondrial DNA depletion and deletions." dans: American journal of physiology. Gastrointestinal and liver physiology, Vol. 301, Issue 1, pp. G119-27, (2011) (PubMed).

Détails sur PRKAA1/PRKAA2

Antigène: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Abstract: PRKAA1/PRKAA2 Produits

Synonymes: anticorps AMPK, anticorps AMPK2, anticorps AMPKa2, anticorps PRKAA, anticorps Ampk, anticorps Ampka2, anticorps 2310008I11Rik, anticorps A830082D05, anticorps AMPKalpha2, anticorps protein kinase AMP-activated catalytic subunit alpha 2, anticorps protein kinase, AMP-activated, alpha 2 catalytic subunit, anticorps PRKAA2, anticorps Prkaa2

Sujet: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-COA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-COA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.