PLK4 anticorps (N-Term)

N° du produit ABIN184701

Détail du produit anti-PLK4 anticorps

Antigène: PLK4 (Polo-Like Kinase 4 (PLK4))

Épitope: N-Term

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PLK4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Fonction: Sak / STK18 / PLK4

Séquence: ATCIGEKIED FKVGN

Homologie: Human, Dog, Cow

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence ATCIGEKIEDFKVGN, from the N Terminus of the protein sequence according to NP_055079.3.

Isotype: IgG

Information d'application

Indications d'application:

DS WB Results: Approx 110 kDa band observed in Human Colon lysates (calculated MW of 109 kDa according to NP_055079.3). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur PLK4

Antigène: PLK4 (Polo-Like Kinase 4 (PLK4))

Autre désignation: PLK4 (PLK4 Produits)

Synonymes: anticorps CG7186, anticorps DmSAK, anticorps Dmel\\CG7186, anticorps PLK4, anticorps Plk4, anticorps SAK/PLK, anticorps SAK/PLK4, anticorps Sak, anticorps plk4, anticorps sak, anticorps SAK, anticorps STK18, anticorps 1700028H20, anticorps AI385771, anticorps Stk18, anticorps PLK-4, anticorps stk18, anticorps wu:fi33g07, anticorps Sak kinase, anticorps polo like kinase 4, anticorps polo-like kinase 4, anticorps polo-like kinase 4 (Drosophila), anticorps serine/threonine-protein kinase PLK4, anticorps SAK, anticorps PLK4, anticorps Plk4, anticorps plk4, anticorps LOC5571876

Classe de substances: Phage Protein

Sujet: PLK4, Sak, polo-like kinase 4 (Drosophila), serine/threonine kinase 18, SAK, Snk Akin Kinase, polo-like kinase 4, protein-serine/threonine kinase, STK18, serine/threonine protein kinase SAK, MCCRP2, STK 18

ID gène: 10733

NCBI Accession: NP_055079, NP_001177728

Pathways: M Phase