PPARG anticorps (AA 280-475)

N° du produit ABIN1860285

Détail du produit anti-PPARG anticorps

Antigène: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Épitope: AA 280-475

Reactivité: Lapin

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Cet anticorp PPARG est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Séquence: MGHHHHHHSG SEF- F QGCQFRSVEA VQEITEYAKN IPGFVSLDLN DQVTLLKYGV HEIIYTMLAS LMNKDGVLIS EGQGFMTREF LKSLRKPFGD FMEPKFEFAV KFNALELDDS DLAIFIAVII LSGDRPGLLN VKPIEDIQDN LLQALELQLK LNHPEASQLF AKLLQKMTDL RQIVTEHVQL LQVIKKTETD MSLHPLLQEI YKDLY

Specificité: It has been selected for its ability to recognize PPARg in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogène: PPARg (AA 280-475)

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Commentaires:

Content: The quality control contains recombinant PPARg (Phe280~Tyr475) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles

Stock: 4 °C

Stockage commentaire: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Date de péremption: 12 months

Détails sur PPARG

Antigène: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Autre désignation: Peroxisome Proliferator Activated Receptor Gamma (PPARg) (PPARG Produits)

Synonymes: anticorps PPAR gamma, anticorps PPARg2, anticorps PPARG, anticorps Nr1c3, anticorps PPAR-gamma, anticorps PPAR-gamma2, anticorps PPARgamma, anticorps PPARgamma2, anticorps NR1C3, anticorps CIMT1, anticorps GLM1, anticorps PPARG1, anticorps PPARG2, anticorps xPPAR-gamma, anticorps PPAR-GAMMA, anticorps PPARGAMMA, anticorps peroxisome proliferator-activated receptor gamma, anticorps peroxisome proliferator activated receptor gamma, anticorps peroxisome proliferator activated receptor gamma L homeolog, anticorps PPARG, anticorps Pparg, anticorps pparg.L

Pathways: Signalisation MAPK, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation